Molecular identification and diversity analysis of dental bacteria in diabetic and non-diabetic females from Saudi Arabia

Publication date: Available online 22 October 2019Source: Saudi Journal of Biological SciencesAuthor(s): Mohammad I. Al-Obaida, Alaa K.M. Al-Nakhli, Ibrahim A. Arif, Asmaa Faden, Sahar Al-Otaibi, Bushra Al-Eid, Aishah Ekhzaimy, Haseeb A. KhanAbstractPeriodontal disease is a chronic infectious disease, which is characterized by the damaged dental hard tissue by lactic acid generated by microorganisms after the fermentation of carbohydrates rich diet. The risk of periodontal disease is known to be higher in diabetic patients. We compared the diversity of five commonly occurring dental bacteria including Porphyromonas gingivalis, Tannerella forsythia, Capnocytophaga ochracea, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans in 14 type-2 diabetic patients and equal numbers of healthy controls. The subgingival samples were collected using sterile paper points. We used 16S rRNA sequence specific primers for PCR-based identification of dental bacteria. Our results showed that A. actinomycetemcomitans was completely absent in control subjects but present in 43% of diabetic patients. C. ochracea was highly prevalent in diabetic patients (100%) as compared to controls (28.5%). The frequency of other three bacterial species was also higher in diabetic patients than control subjects. These findings indicate that dental bacteria are highly prevalent in subgingival pockets of diabetic patients. Therefore, proper monitoring of diabetic patients for dental care is important t...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research