Genes, Vol. 10, Pages 836: Deregulated Adhesion Program in Palatal Keratinocytes of Orofacial Cleft Patients

Genes, Vol. 10, Pages 836: Deregulated Adhesion Program in Palatal Keratinocytes of Orofacial Cleft Patients
Genes doi: 10.3390/genes10110836
Authors:
Mammadova
Carels
Zhou
Gilissen
Helmich
Bian
Zhou
Von den Hoff

Orofacial clefts (OFCs) are the most frequent craniofacial birth defects. An orofacial cleft (OFC) occurs as a result of deviations in palatogenesis. Cell proliferation, differentiation, adhesion, migration and apoptosis are crucial in palatogenesis. We hypothesized that deregulation of these processes in oral keratinocytes contributes to OFC. We performed microarray expression analysis on palatal keratinocytes from OFC and non-OFC individuals. Principal component analysis showed a clear difference in gene expression with 24% and 17% for the first and second component, respectively. In OFC cells, 228 genes were differentially expressed (p < 0.001). Gene ontology analysis showed enrichment of genes involved in β1 integrin-mediated adhesion and migration, as well as in P-cadherin expression. A scratch assay demonstrated reduced migration of OFC keratinocytes (343.6 ± 29.62 μm) vs. non-OFC keratinocytes (503.4 ± 41.81 μm, p < 0.05). Our results indicate that adhesion and migration are deregulated in OFC keratinocytes, which might contribute to OFC pathogenesis.

Source: Genes - October 22, 2019 Category: Genetics & Stem Cells Authors: Mammadova Carels Zhou Gilissen Helmich Bian Zhou Von den Hoff Tags: Article Source Type: research