LncRNA CRNDE regulates trophoblast cell proliferation, invasion, and migration via modulating miR-1277.

This study aims to investigate the functional role of LncRNA CRNDE in PE pathogenesis and its effects on trophoblasts. mRNA levels of CRNDE and miR-1277 in placenta tissues and HTR-8/SVneo cells were analyzed by qRT-PCR. Western blot was carried out to evaluate protein level of E-cadherin, β-Catenin, Vimentin, MMP2 and MMP9. Cell proliferation was assessed by using CCK-8 assay and colony formation assay, whereas cell migration and invasion were evaluated by wound healing assay and transwell assay, respectively. Moreover, the interrelation between CRNDE and miR-1277 was verified by dual luciferase reporter assay. The results showed that CRNDE expression was significantly downregulated, whereas miR-1277 expression was significantly upregulated in PE placental tissues. Overexpression of CRNDE facilitated HTR-8/SVneo cell proliferation, migration and invasion, promoted the EMT formation, and increased the protein expression of MMP2 and MMP9, while knockdown of CRNDE had the opposite results. In addition, we also found that miR-1277 was negatively regulated by CRNDE and was a direct target of CRNDE. Furthermore, knockdown of miR-217 promoted HTR-8/SVneo cell proliferation, migration, and invasion, while knockdown of CRNDE reversed the accelerating effects of miR-1277 on HTR-8/SVneo cells. LncRNA CRNDE may suppress trophoblast cell proliferation, migration and invasion at least partly through regulating miR-1277 in trophoblast cells. CRNDE is promising to function as a new tar...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research

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[USAID] On December 27 and December 31, 2019, the U.S Agency for International Development (USAID) launched the first two in a series of global health awards to accelerate reductions in maternal, newborn, and child mortality and morbidity in high-burden countries.
Source: AllAfrica News: Pregnancy and Childbirth - Category: OBGYN Source Type: news
Source: Journal of Maternal-Fetal and Neonatal Medicine - Category: Perinatology & Neonatology Authors: Source Type: research
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Source: African Health Sciences - Category: African Health Source Type: research
Conclusion: Due to general lack of awareness towards toxoplasmosis observed among both health workers and pregnant women in Temeke Municipality, we recommend health policy on maternal and child healthcare to address prenatal screening that is aimed at providing early diagnosis for any possible congenital toxoplasmosis as well as diseases that are currently screened in Tanzania such as HIV, syphilis and malaria. Integrating a One Health approach in educating medical professionals and the vulnerable population of pregnant women on the importance of congenital zoonoses will promote awareness and preventive practices towards t...
Source: African Health Sciences - Category: African Health Source Type: research
Background: Stillbirth is a major adverse perinatal outcome especially in low and middle income countries across the globe. Certain factors relating to mothers from such countries may be associated with this adverse condition.Objectives: To determine the prevalence of stillbirth and also explore the maternal socio-demographic factors associated with stillbirth among mothers in rural communities in Anambra Central Senatorial District of Anambra State Nigeria who gave birth between January 2012 and December 2016.Methods: All case files of mothers who were delivered of their babies were accessed at the sampled health faciliti...
Source: African Health Sciences - Category: African Health Source Type: research
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Source: Reproductive Toxicology - Category: Toxicology Source Type: research
The Trump administration is coming out with new visa restrictions aimed at restricting a practice known as “birth tourism
Source: ABC News: Health - Category: Consumer Health News Tags: Health Source Type: news
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Publication date: Available online 22 January 2020Source: European Journal of Obstetrics &Gynecology and Reproductive BiologyAuthor(s): Amihai Rottenstreich, Misgav Rottenstreich, Zvi Ehrlich, Yosef Ezra, David Mankuta, Simcha Yagel, Doron Kabiri, Uriel Elchalal, Gabriel LevinAbstractObjectiveHistory of prior preterm birth (PTB) represents one of the strongest risk factors for recurrent PTB. Nevertheless, whether the occurrence of PTB in multifetal gestation is associated with increased risk of PTB in subsequent pregnancies remains unclear. We aimed to determine the recurrence risk of PTB in a subsequent singleton preg...
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
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