Label-free liquid biopsy based on blood circulating DNA detection using SERS-based nanotechnology for nasopharyngeal cancer screening

Publication date: Available online 21 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Duo Lin, Qiong Wu, Sufang Qiu, Guannan Chen, Shangyuan Feng, Rong Chen, Haishan ZengAbstractDevelopment of a sensitive, rapid and easy-to-use liquid biopsy method is of imperative clinical value for point-of-care caner diagnostics. Here, a label-free and modification-free nanotechnology based on surface-enhanced Raman spectroscopy (SERS) was employed for DNA analysis. Using the SERS signals of phosphate backbone as internal standard, quantitative detection for nucleobases was achieved even at single base level. The method combined with principal component analysis and linear discriminant analysis was further applied for real blood circulating DNA detection for the first time, and an ideal diagnostic sensitivity of 83.3% and specificity of 82.5% could be obtained for differentiating the nasopharyngeal cancer from the normal group, demonstrating promising potential as an alternative nanotechnology for nasopharyngeal cancer screening based on liquid biopsy.Graphical AbstractIn present work, a label-free and modification-free detection method using silver nanoparticles-based surface-enhanced Raman spectroscopy was developed for DNA analysis. The small changes in DNA molecules can be rapidly and sensitively quantified at single nucleobase level without complicated procedures. Moreover, this method combined with diagnostic algorithm was employed for blood circulati...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research