Purification of Hyaluronidase as an anti-cancer agent inhibiting CD44.

Purification of Hyaluronidase as an anti-cancer agent inhibiting CD44. Biomed Chromatogr. 2019 Oct 19;:e4709 Authors: Shakouri A, Parvan R, Adljouy N, Abdolalizade J Abstract Hyaluronidase (Hyal) can be employed to accomplish a diversity of complications related to Hyaluronic acid (HA). Hyal contains some classes of catalysts that cleave HA. This enzyme is detected in several human tissues as well as in animal venoms, pathogenic organisms and cancers. Destructive cancer cells regularly increase the CD44 receptor existing in a cell membrane. This receptor acts as an exact receptor for HA, and HA is recognized to motivate migration, spreading, attack, and metastasis of cancer cells. Nearly all the methods used to purify Hyal are highly costly and not proper for industrial applications. This survey aims to review different methods of Hyal purification, which acts as an anti-cancer agent by degrading HA in tissues and thus inhibiting the CD44-HA interaction. Hyal can be successfully employed in the management of cancer, which is associated with HA- CD44. Reviews have described purification methods for Hyal to prepare an origin to develop a novel purification technique for this highly appreciated protein. Using multiple columns is not applicable for the purification of Hyal and thus cannot be used in the industrial level. It is better to use affinity chromatography of anti-Hyal for Hyal with one-step purification. PMID: 31630417 [...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research