Application of one –factor- at-a-time and statistical designs to enhance α-amylase production by a newly isolate Bacillus subtilis strain-MK1

Publication date: Available online 20 October 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Samia A. Ahmed, MohamedA.A. Abdella, Gamal M. El-Sherbiny, Atef M. Ibrahim, Aliaa R. El-Shamy, SherienM.M. AtallaAbstractα-amylase is a starch hydrolyzing enzyme which has many industrial applications. In the present study, α-amylase producers were isolated from farm soil in Egypt (Sadat City). Screening was done by iodine test, based on the clear zone around the sample in starch agar plates. The most potent isolate MK1 was identified (morphologically and biochemically) and confirmed by 16S rRNA gene sequence method. This sequence was deposited to the GenBank under accession number, B. subtilis strain-MK1 (MF614924). α-Amylase production parameters were optimized using one-factor-at-a-time (OFAT) and Response Surface methodology (RSM). The result obtained from OFAT showed 72.4U/ml of amylase activity which was 1.8- fold higher as compared to unoptimized conditions (40.3 U/ml). The most significant factors were identified and its values were optimized using Plackett-Burman (PB) and Central Composite (CC) design, respectively. Among thirteen independent variables tested in PB design, beef extract, MgSO4·7H2O, K2HPO4 and incubation time were the most significant on α-amylase production and showed positive effect. Whereas MnCl2·4H2O, soluble starch and culture pH showed negative effect on α-amylase production. The model valida...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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This study demonstrates the great potential of machine learning in electrochemical researches.Graphical abstract
Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
Publication date: Available online 9 November 2019Source: Journal of Electroanalytical ChemistryAuthor(s): Kanjana Kunpatee, Phuktra Chamsai, Eda Mehmeti, Dalibor M. Stankovic, Astrid Ortner, Kurt Kalcher, Anchalee SamphaoAbstractA sensitive and rapid method for the determination of fenobucarb by using screen-printed carbon electrode modified with graphene nanoribbons - ionic liquid - cobalt phthalocyanine (GNRs-IL-CoPc/SPCE) composites based on flow injection analysis (FIA) was developed and applied to vegetable samples. The prepared GNRs-IL-CoPc composite was characterized by Fourier transform infrared spectroscopy (FT-I...
Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
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Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
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Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
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Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
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Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
In this study, 2 mol% Co(NO3)2•6H2O was used as the sintering aid, GDC interlayers with 2 mol% Co(NO3)2•6H2O (Co-GDC) were sintered at 1150 °C, 1200 °C, 1250 °C and 1350 °C, respectively. The Co-GDC interlayer sintered at 1150 °C was porous, but only closed pores existed when the Co-GDC interlayers were sintering above 1250 °C. The initial ohmic resistances of the cells with Co-GDC interlayers sintered at 1150 °C, 1250 °C and 1350 °C were 0.259, 0.266 and 0.299 Ω cm2. After the cells running for 100 h, the ohmic resistances were 0.289, 0.262 an...
Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
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Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
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