Development of highly sensitive and selective sensor for ethionamide guided by molecular modelling via electropolymerized molecularly imprinted films
Publication date: Available online 20 October 2019Source: Microchemical JournalAuthor(s): Archana Kushwaha, Neha Gupta, Juhi Srivastava, Ambareesh Kumar Singh, Meenakshi SinghAbstractHere, a selective sensing technology for the drug ethionamide used to treat multi-drug resistant tuberculosis is developed based on molecular imprinting. The molecular imprinting matrix is developed strategically by molecular modelling via density functional theory. 3-Thiophene acetic acid was predicted as suitable functional monomer for imprinting ethionamide. Polymeric film was formed on the surface of glassy carbon electrode by electropolymerization of 3-thiophene acetic acid in presence of ethionamide as template by cyclic voltammetry. The obtained sensor was highly selective towards analyte ethionamide and the recognition was quantified by differential pulse voltammetric experiments of ethionamide molecule. A highly selective (imprinting factor: 8.92) and sensitive (limit of detection: 0.005 µg/mL) molecularly imprinted sensor with good reproducible behaviour (R2 = 0.9882, RSD 2.68%) was fabricated.
Publication date: Available online 18 November 2019Source: Journal of Microbiological MethodsAuthor(s): Guocan Yu, Yanqin Shen, Bo Ye, Da Chen, Kan XuAbstractThe CapitalBio™ Mycobacterium nucleic acid detection test is a real-time fluorescent polymerase chain reaction and TaqMan probe technology test for rapid diagnosis of extrapulmonary tuberculosis (EPTB). This test had moderate sensitivity and high specificity for EPTB, like that of Xpert MTB/RIF, especially for lymph node, chest wall, and purulent fluid samples.
Authors: Van Slambrouck J, Vlasselaers J, Devos B Abstract Peritoneal tuberculosis is a rare extrapulmonary manifestation of tuberculosis. Considering the diagnosis of peritoneal tuberculosis in a low prevalence setting can be vital for the further clinical management in selected patients.In a young male with migration background, presenting with generalised abdominal pain, computed tomography revealed ascites, omental and peritoneal thickening and enlarged mesentery lymph nodes. Computed tomography also revealed a distal ureteral calculus at the right vesico-ureteral junction. Diagnostic laparoscopy showed a nodul...
We describe 2 cases of miliary tuberculosis in young children with clinically unexpected central nervous system involvement. Magnetic resonance imaging of the brain should be considered part of the initial diagnostic workup for miliary tuberculosis in very young children.
Microbiologic confirmation of pediatric extrapulmonary tuberculosis remains challenging, leading to diagnostic delays. In our retrospective case series, real-time molecular testing (Xpert MTB/RIF) on respiratory and extrapulmonary specimens resulted in a more rapid diagnosis of extrapulmonary tuberculosis in a nonendemic, high resource setting.
Abstract Used as a ventilator for assisting victims of polio, the barospirator was described by Swedish physician-scientist Torsten Thunberg in 1924. An immediate predecessor of the iron lung of Philip Drinker, the barospirator fully encased the entire body. Cyclic air-pressure changes within the chamber achieved ventilation during equilibrations of intrapulmonary and ambient pressures. Pulmonary medicine innovator Alvan Leroy Barach used a modified barospirator for lung rest as a treatment of tuberculosis in the 1940s. Adverse effects included damage to patients' tympanic membranes. Despite its limited clinical s...
(Trinity College Dublin) By understanding how, when, and why badgers move from one social group to another, researchers hope information gleaned from GPS devices will help them tailor vaccination programs to reduce the spread of bovine tuberculosis.
Authors: Araujo-Mariz C, Militão de Albuquerque MFP, Lopes EP, Ximenes RAA, Lacerda HR, Miranda-Filho DB, Lustosa-Martins BB, Pastor AFP, Acioli-Santos B Abstract INTRODUCTION AND OBJECTIVE: Hepatotoxicity during tuberculosis (TB) treatment is frequent and may be related to the Arylamine N-Acetyltransferase (NAT2) acetylator profile, in which allele frequencies differ according to the population. The aim of this study was to investigate functional polymorphisms in NAT2 associated with the development of hepatotoxicity after initiating treatment for TB in people living with HIV/AIDS (PLWHA) in Pernambuco, Nor...
Conditions: Hiv; Tuberculosis Intervention: Drug: Dolutegravir Sponsors: Helen Reynolds; Infectious Disease Institute, Kampala, Uganda; Desmond Tutu HIV Centre Not yet recruiting