Theranostic nanozyme: Silk fibroin based multifunctional nanocomposites to combat oxidative stress

Publication date: February 2020Source: Materials Science and Engineering: C, Volume 107Author(s): Mehak Passi, Vinay Kumar, Gopinath PackirisamyAbstractMultifunctional nanomaterials integrating therapeutic and imaging modalities in one platform have opened a new era in the present therapeutic strategies. In the present study, a multifunctional silk fibroin-based carrier has been designed for the delivery of antioxidant and imaging agents. One-step desolvation method was used to prepare sulforaphane (antioxidant drug) loaded silk fibroin nanoparticles (SFSNPs). These anionic SFSNPs were further coupled with cationic cerium oxide nanoparticles (CeNPs) and PEI passivated carbon dots (CDs) to form self-assembled CeNP-CD@SFSNPs nanocomposites. CDs were synthesized from mulberry leaves (Morus indica) as green source of carbon and bPEI as a passivating agent to get positively charged CDs. The CDs functioned as molecular probes by emitting green fluorescence while the presence of CeNPs augmented the antioxidant potential due to their unique redox property. Time-dependent in vitro release of sulforaphane was fast in acidic pH than under normal physiological conditions. Cytotoxicity studies were performed on L132 normal epithelial lung cell lines and A549 lung cancer cell lines to analyze the toxicity of the nanocomposites. Green fluorescence from the CDs facilitated in fluorescence microscopic imaging and cellular uptake studies. ROS scavenging capability was analyzed by exposing cell...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research

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Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research
Standardized Nucleic Acid Quantification for SEQuencing (SNAQ-SEQ) is a novel method that utilizes synthetic DNA internal standards spiked into each sample prior to next generation sequencing (NGS) library pre...
Source: BMC Cancer - Category: Cancer & Oncology Authors: Tags: Technical advance Source Type: research
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Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research
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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
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Source: Life Sciences - Category: Biology Source Type: research
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Source: Cellular Signalling - Category: Cytology Authors: Tags: Cell Signal Source Type: research
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Source: Pharmacological Research - Category: Drugs & Pharmacology Source Type: research
Ding-Kun Zhang Gac fruit (Momordica cochinchinensis Spreng) is a popular tropical fruit in southeast Asia. What is amazing is that its seeds (Momordicae Semen) and arils are traditional herbs with anti-tumor activity, and have protected human health for more than 1000 years. In recent years, its anti-tumor activity has received extensive attention and research. This manuscript summarized the chemical composition of saponins, fatty acids, volatile constituents, proteins, peptides, and other components from Momordicae Semen (MSE). The effect and mechanism of MSE and its extract on breast cancer, gastric cancer, lung c...
Source: Molecules - Category: Chemistry Authors: Tags: Review Source Type: research
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Source: International Journal of Molecular Medicine - Category: Molecular Biology Authors: Tags: Int J Mol Med Source Type: research
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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
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