Comprehensive identification of steroid hormones in human urine based on liquid chromatography-high resolution mass spectrometry.

Comprehensive identification of steroid hormones in human urine based on liquid chromatography-high resolution mass spectrometry.
Anal Chim Acta. 2019 Dec 16;1089:100-107
Authors: Zheng Y, Zhao H, Zhu L, Cai Z
Abstract
Steroid hormones, structural derivatives of cyclopentanoperhydrophenanthrene, play important roles in modulation of many physiological processes. Comprehensive characterization of steroid hormones is valuable for understanding the process of human life activities and even disease diagnosis. Hitherto systematical characterization of steroid hormones has been rarely investigated. Here, we presented an integrated method for human urine analysis based on ultra-high performance liquid chromatography-high resolution mass spectrometry in data-dependent acquisition mode with the following parallel reaction monitoring mode. To process the data acquired by two scan modes, a comparative study of standards' fragmentation behaviors and diagnostic product ions (DPIs) were firstly conducted to facilitate the characterization of steroid hormones. The fragmentation behaviors, DPIs, elemental composition and double-bond equivalent were then simultaneously utilized for systematical characterization of steroid hormones in human urine. Consequently, fragmentation pathways and DPIs for all types of steroid hormones were comprehensively interpreted. It is interesting to find that dehydration is not restricted in the form of hydroxyl groups l...

Source: Analytica Chimica Acta - October 21, 2019 Category: Chemistry Authors: Zheng Y, Zhao H, Zhu L, Cai Z Tags: Anal Chim Acta Source Type: research