Characterization of zinc oxide nanoparticles-epoxy resin composite and its antibacterial effects on spoilage bacteria derived from silvery pomfret (Pampus argenteus)

Publication date: December 2019Source: Food Packaging and Shelf Life, Volume 22Author(s): Jiahao Xu, Ru Song, Yubin Dai, Shuyi Yang, Jie Li, Rongbian WeiAbstractEpoxy resin was conjugated with zinc oxide nanoparticles (ZnO NPs) to prepare antibacterial zinc oxide-epoxy resin (ZnO-ER) composite. The property of ZnO-ER was characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and SEM-energy dispersive X-ray spectroscopy (SEM-EDS) assays. Results showed the presence of ZnO NPs on the surface and cross-sections of ZnO-ER composite. The average thickness of ZnO-ER was 392.38 μm. The strong antibacterial effect of ZnO-ER on the test bacterial mixture derived from spoiled silvery pomfret (Pampus argenteus) was observed using SEM assay. After addition of ZnO-ER for 6 h in the bacterial mixture, cellular membrane integrities of most bacteria were destroyed. High-throughput 16S ribosomal RNA gene sequencing revealed the decreases of both community richness and diversity in the ZnO-ER treated bacterial mixture. As for relative abundances, significant decreases were detected in the phyla Bacteroidetes and Firmicute, while dramatic increase of the phylum Proteobacteria was observed after ZnO-ER treatment. The family of Enterobacteriaceae was predominant in the ZnO-ER treatment. The genera of Providencia and Yersinia showed dramatically high relative abundances. However, the abundances of some specific genera such as Aeromonas and Shewanell, which are related t...
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research