An immunomodulatory feed additive enhances in vitro viral vaccine recall antigen responses in dairy heifers

The objective of this study was to evaluate post-vaccine antibody titers and circulating cellular memory development in heifers fed OG and administered a commercially available modified-live bovine respiratory disease (BRD) vaccine. Twenty-four Holstein heifers were assigned to one of two diets for 170 days: Control TMR (CON; n = 11), or TMR plus OG (TRT; 9 g/100 kg BW/day; n = 13). Samples for hematology, serology, and cellular assays were collected on D-110, 0, 21, 42, and 60 of the trial. Heifers were administered two priming doses of a modified-live BRD vaccine, with a third dose given on D0. There were no significant differences in total WBC and absolute number or the percentage of circulating lymphocytes, monocytes, neutrophils, RBC, or platelets on D-110 through D21. On D42 and D60, CON had significantly higher numbers of lymphocytes. On D0, mean serum neutralizing (SN) titer to BHV-1 was significantly higher for CON compared to TRT. SN titers were not significantly different between CON and TRT at any other time point for BHV-1, BVDV type 1, or BVDV type 2. TRT mounted a significantly stronger recall proliferative response to 0.5 multiplicity of infection (MOI) of BHV-1, BVDV type 1 and BVDV type 2 on D42 and D60; 0.25 MOI of BVDV type 1 on D21 and D42; and 0.25 MOI BVDV type 2 on D42 compared to CON. IL-4 production induced by 0.5 and 1.0 MOI BHV-1 (D42 and D60); 0.25 MOI of BVDV type 1 (D21); and 0.25 and 0.5 MOI of BVDV type 2 (D60) were significantly...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research

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This study not only determined the primary reason for the atypical IBD epidemic, but also remind us of the urgency for developing new vaccines against novel variant IBDV.
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 15 November 2019Source: Veterinary MicrobiologyAuthor(s): Xian-Wei Wang, Mi Wang, Jing Zhan, Qian-Yu Liu, Lin-lin Fang, Chen-yao Zhao, Ping Jiang, Yu-Feng Li, Juan BaiAbstractSince late 2010, highly virulent PEDV G2-genotype strains have emerged globally extracting heavy losses on the pork industries of numerous countries. We investigated the characteristics of a field strain of PEDV (PEDV strain SH) isolated from a piglet with severe diarrhea on a farm in Shanghai China. Whole genome sequencing and analysis revealed that the SH strain belonged to subtype G2a and has a unique 12-aa deleti...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
In this study we demonstrate that this vaccine provides, two weeks after vaccination, an additional protection (prevention of mortality, clinical signs, renal infection, bacterial excretion, renal carriage and renal lesions) against fatal leptospirosis due to Leptospira interrogans serovar Copenhageni (serovar of major medical importance).
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research
ConclusionWe review the current information on the causative factors and mechanisms of CD, including smoking, exercise, diet, animal protein, breastfeeding, history of childhood infection and vaccination, oral contraceptives, and antibiotics of CD. However, further studies are needed to understand knowledge gaps in the field of CD.
Source: International Journal of Colorectal Disease - Category: Gastroenterology Source Type: research
Publication date: Available online 14 November 2019Source: Protein Expression and PurificationAuthor(s): Haruna Yuasa, Eiji Kotani, Hajime Mori, Keiko TakakiAbstractCypovirus is an insect virus that is encapsulated in stable cubic protein crystals composed of polyhedrin protein produced in virus-infected cells. Molecular technology developed over the last decade is now able to immobilise proteins of interest on polyhedrin crystals. Modified polyhedrin crystals can be used in cell cultures for implantation in animals and vaccines, among other applications. However, this technique does not work for some proteins. Here, we de...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
AbstractIn Xayaboury province, located in the northern region of Lao PDR, the foot and mouth disease (FMD) vaccination campaign just began in 2009. Up until now, a small number of farms have been vaccinated. When FMD outbreaks occur, it is interesting to determine the risk factors of FMD, especially in the area where vaccination rates are low. The questionnaire survey, using a case-control design at the household level, was carried out. From 59 villages with a total number of 434 households, 181 households who experienced FMD were assigned as case households, 146 households without FMD occurrence inside the outbreak villag...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
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Source: OnMedica Latest News - Category: UK Health Source Type: news
AbstractSheep brucellosis is a worldwide extended disease caused byB. melitensis andB. ovis, two species respectively carrying smooth or rough lipopolysaccharide. VaccineB. melitensis Rev1 is used againstB. melitensis andB. ovis but induces an anti-smooth-lipopolysaccharide response interfering withB. melitensis serodiagnosis, which precludes its use againstB. ovis whereB. melitensis is absent. In mice, Rev1 deleted inwbkC (Brucella lipopolysaccharide formyl-transferase) and carryingwbdR (E. coli acetyl-transferase) triggered antibodies that could be differentiated from those evoked by wild-type strains, was comparatively ...
Source: Veterinary Research - Category: Veterinary Research Source Type: research
Germany's Boehringer Ingelheim, the global No. 2 in animal health, is having to rebuild itself in China after a deadly pig disease decimated sales of unrelated mainstay vaccines, bringing write-offs and leaving it facing a three-year slog to recovery.
Source: Reuters: Health - Category: Consumer Health News Tags: healthNews Source Type: news
Publication date: Available online 13 November 2019Source: Veterinary MicrobiologyAuthor(s): Linlin Li, Yun Zhang, Jiawen Dong, Junqing Zhang, Chunhong Zhang, Minhua Sun, Yongchang CaoAbstractDuck Tembusu virus (DTMUV) is a major pathogen of duck industry in China. In the current study, we generated different constructs containing envelope (E) protein, pre-membrane-envelope (prM-E) protein, and C-terminally truncated E protein of the DTMUV. The constructed proteins could induce specific antibody responses in young ducks. When ducklings were immunized with the constructed proteins, they were 100% protected against DTMUV inf...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
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