The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse

Publication date: Available online 19 October 2019Source: Enzyme and Microbial TechnologyAuthor(s): Lorena Cardoso Cintra, Isabelle Cristine da Costa, Izadora Cristina Moreira de Oliveira, Amanda Gregorim Fernantes, Syd Pereira Faria, Rosália Santos Amorin Jesuíno, Maria Cristina Ravanal, Jaime Eyzaguirre, Luiz Pereira Ramos, Fabrícia Paula de Faria, Cirano José UlhoaAbstractTo increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required. Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-L-arabinofuranosidases (ABF) is necessary. The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB). One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-L-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD). The most potent enzyme for SEB hydrolysis was ABF3. Subsequently, the optimal enzyme mixture was used in combination w...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research

Related Links:

For 22 years, the world's largest pure-play medical device company was the only player in sacral neuromodulation, a space that addresses urinary problems as well as fecal incontinence. Now there's a new kid on the block, which has created quite an intense David vs. Goliath medtech story. For those unfamiliar with the Axonics vs. Medtronic story up to this point, click here for a quick rundown of recent events that have transpired in this market.   FDA Approves Second Indication for Axonics r-SNM FDA approved the urinary indications for an implantable rechargeable sacral neuro...
Source: MDDI - Category: Medical Devices Authors: Tags: Business Implants Source Type: news
Publication date: Available online 16 November 2019Source: Materials Science and Engineering: AAuthor(s): Zeju Weng, Kaixuan Gu, Kaikai Wang, Xuanzhi Liu, Junjie WangAbstractDeep cryogenic treatment (DCT) is believed to have superior performance in coordination with traditional heat treatment. In present work, the effect of DCT combined with inter-critical quenching treatment on the mechanical properties and microstructure of 30CrMnSi alloy structural steel was investigated. Before DCT employed, quenching treatment was conducted at different austenitizing temperatures (780 °C, 820 °C and 900 °C) to obtain...
Source: Materials Science and Engineering: A - Category: Materials Science Source Type: research
Publication date: Available online 16 November 2019Source: Materials Chemistry and PhysicsAuthor(s): M. Ramadas, Khalil Ei Mabrouk, A.M. BallamuruganAbstractThree-dimensional (3D) porous apatite (HAP) scaffold has recently emerging as functional biomaterials in bone tissue engineering and wound healing. Here we present a study of the preparation of apatite (HAP) scaffold by gel-casting technique. The scaffold prepared as engineering constructs with non-uniform porosity and interconnected pores with a micro size of 2  μm–2.4 μm range. The resultant scaffolds were characterized in terms of crystalline phase...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
In this study, we determined the initial freezing temperatures (Tf) and glass transition temperatures (Tg) of pumpkin using differential scanning calorimetry at various water contents. The vapor sorption analyzer was used to determine water sorption properties while spectrophotometer used to measure color changes in pumpkin after 13 months of storage. We constructed a state diagram using Tf and Tg; and determined critical water activity from Tg, water content and water activity diagram. The Tf decreased from −1.3 to −21.0 °C as the solids content increased from 0.08 to 0.63 g solid/g pumpkin. The Tg dec...
Source: Journal of Food Engineering - Category: Food Science Source Type: research
Publication date: Available online 16 November 2019Source: Thermochimica ActaAuthor(s): Yi-jian He, Shu-peng Zheng, Guang-ming ChenAbstractA modified heat conduction calorimeter was developed and utilized in experiments using a quasi-scanning method. The isobaric heat capacities of R143a and R227ea in the liquid phase were measured at a maximum pressure over 5 MPa and temperatures of 310.15 K-365.15 K, respectively. The relatively expanded uncertainties of the measurements were estimated to be 0.030 and 0.029 for R143a and R227ea, respectively.The measurements resulted in 27 data points for R143a and 5...
Source: Thermochimica Acta - Category: Chemistry Source Type: research
Publication date: Available online 17 November 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Lauro A. Pradela-Filho, Isabela A.A. Andreotti, Jefferson H.S. Carvalho, Diele A.G. Araújo, Luiz O. Orzari, Alexandre Gatti, Regina M. Takeuchi, André L. Santos, Bruno C. JanegitzAbstractNowadays, there is growing interest in portable and disposable electrochemical sensors due to the low-cost, simplicity, and excellent analytical performance associated with these devices. Despite recent advances, however, there remains a need to create cheap, high-performance electrode materials. Thus, this study evaluated t...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
Publication date: Available online 16 November 2019Source: Journal of Electroanalytical ChemistryAuthor(s): A. Dennyson Savariraj, V. Vinoth, R.V. Mangalaraja, T. Arun, David Contreras, Ali Akbari-Fakhrabadi, Héctor Valdés, Fawzi BanatAbstractThree-dimensional (3D) bismuth selenide (Bi2Se3) nanostructures were synthesized by microwave synthesis using water as a solvent and hydrazine hydrate as a reducing agent and exfoliated into few layers of Bi2Se3. The Bi2Se3- Few Layers (Bi2Se3- FL) exhibited localized surface plasmon resonance and enhanced electrocatalytic behavior. The scanning electron microscope (SEM)...
Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
ConclusionWithin the limitations of the study, GBR with or without autogenous block graft may be an effective approach in augmenting horizontally deficient mandibular or maxillary ridges, before the placement of dental implants. However, more complications may be seen with the use of an autogenous block graft related to the donor sites.
Source: Journal of Evidence Based Dental Practice - Category: Dentistry Source Type: research
In conclusion, this study presents the novel finding that the PVS periductium is composed of squamous MCs. These cells tightly line the luminal surface of the PVS tissue, including PNs, PVs, and small branches of the PVs in the abdominal cavity. These results will help researchers understand the role of the PVS in hyaluronan secretion, tissue repair, inflammation, and tumorigenesis, as well as the fine structure of PVS tissue.
Source: Journal of Acupuncture and Meridian Studies - Category: Complementary Medicine Source Type: research
Publication date: Available online 16 November 2019Source: Materials Today: ProceedingsAuthor(s): Vijay Kumar Chavan, M. SreenivasuluAbstractVanadium ion doped zinc lead lithium phosphate glass samples are prepared by melt quench method. X-ray diffraction studies confirmed the amorphous nature of all the prepared glasses. The density and refractive index values of these glasses are evaluated using the Archimedes method and Abbe’s refractometer respectively. The values of density found to be in between 3.6363 and 3.6666 g/cm3. The refractive index values are observed in the range 1.5605–1.5620. An increasing t...
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
More News: Biotechnology | Middle East Health | PET Scan