Chitosan-carbon quantum dots as a new platform for highly sensitive insulin impedimetric aptasensor

Publication date: Available online 19 October 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Farbod Abazar, Abdollah NoorbakhshAbstractConsidering the importance of measuring insulin in the prevention and diagnosis of diabetes-related illnesses, here we introduced an ultrasensitive electrochemical aptamer-based sensor for detection of insulin in human blood serum. In this research, carbon quantum dots (CQDs) were synthesized using simple, fast and cheap methods from candle soot. By dispersion of the CQDs in chitosan (Chi) matrix, homogenous CQD-Chit nano-composite was obtained. The experimental results demonstrate the good advantages of proposed CQD-Chit nano-composite, such as homogeneity, high stability and multiple amino groups for covalent immobilization of aptamer and fabrication of highly selective insulin aptasensor. CQD-Chit nano-composite was characterized by the transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDAX), Fourier transform infrared (FTIR) spectroscopy and electrochemical techniques. Under the optimized conditions with electrochemical impedance spectroscopy technique, a linear response ranges from 0.5 nM to 10 nM with the sensitivity of 80.07 Ω/nM and the very low detection limit of 106.8 pM was obtained for insulin detection. The fabricated aptasensor is validated in comparison with standard methods for detection of insulin in human blood...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research

Related Links:

ConclusionsSuvarna Bhasma contained spherical gold nanoparticles. It was nontoxic in rat model at the does tested. Suvarna Bhasma has anxiolytic effects in zebrafish behavioural model.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
CONCLUSION: There was a good agreement for the size of the NVs in both methods. OCTA can be used as a simple and non-invasive method to visualize retinal neovascularizations. PMID: 31711250 [PubMed - in process]
Source: Klinische Monatsblatter fur Augenheilkunde - Category: Opthalmology Tags: Klin Monbl Augenheilkd Source Type: research
AbstractThe accurate 3D reconstruction of organs from radiological scans is an essential tool in computer-aided diagnosis (CADx) and plays a critical role in clinical, biomedical and forensic science research. The structure and shape of the organ, combined with morphological measurements such as volume and curvature, can provide significant guidance towards establishing progression or severity of a condition, and thus support improved diagnosis and therapy planning. Furthermore, the classification and stratification of organ abnormalities aim to explore and investigate organ deformations following injury, trauma and illnes...
Source: Journal of Medical Systems - Category: Information Technology Source Type: research
AbstractIntroductionPregnancy ‐related medical complications are associated with a 2‐ to 5‐fold increased risk of preterm birth (PTB), but the nature of this etiologic relationship in context with maternal factors remains poorly understood. Previous studies have generally treated maternal age as a confounder but overlooked its potential as an effect modifier, whereby the magnitude of the effect of complications on PTB could differ significantly across age groups. We investigated whether advanced maternal age (≥35 years) modified the association between pregnancy complications and PTB, and compared population attri...
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
AbstractIntroductionMidwife ‐led models of care have been subject of debate for many years. We conducted a study to compare intrapartum and neonatal mortality rates in midwife‐led (primary) versus obstetrician‐led (secondary) care at the onset of labour in low risk term women.Material and methodsWe performed an unmatched and a propensity score matched cohort study using data from the national perinatal audit registry (PAN) and from the national perinatal registry (PERINED) of the Netherlands. We included women with singleton pregnancies (without congenital anomalies or antepartum fetal death) who gave birth at term b...
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
ConclusionIn diabetic patients, discrete changes of some parameters in the central ring of the mfERG field map (e.g., P1 and N2 amplitudes) have a significant correlation with both structural OCT abnormalities in the corresponding points of the thickness map (like DRIL, intraretinal cyst and ELM/EZ disruption) and BCVA. Predictive models such as those described in this report may make it possible to identify the relationship between specific anatomical and functional characteristics in diabetic retinopathy.
Source: International Ophthalmology - Category: Opthalmology Source Type: research
This study aim is to prepare a suitable high- performance vector for gene therapy in diabetes mellitus. The designed vector has prominent characteristics, such as directed replacement, which makes it a suitable method for treating or preventing other genetic disorders. Materials and Methods: Whole rDNA sequence of the human genome was scanned. The 800 bp two homology arms were digested by EcoRI and synthesized and cloned into the pGEM-B1 plasmid (prokaryotic moiety). The carbohydrate sensitive promoter, L-pyruvate kinase, and insulin gene were sub-cloned between homologous arms (eukaryotic moiety). The PGEM-B1 plasmid was ...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research
Purpose of review To provide clinicians an overview to the diagnosis, treatment, and management of Paget's disease, including recent guideline recommendations, with comparison of the Endocrine Society Clinical Practice Guideline (JCEM 2014) with the new Clinical Guideline review endorsed by the International Osteoporosis Foundation, the American Society of Bone and Mineral Research, the European Calcified Tissues Society, and the United Kingdom Bone Research Society (JBMR 2019). Recent findings Radionuclide bone scans are recommended for assessing the extent of Paget's disease. Bisphosphonates remain the mainstay for ...
Source: Current Opinion in Endocrinology, Diabetes and Obesity - Category: Endocrinology Tags: SPECIAL COMMENTERIES Source Type: research
(Natural News) Antidepressants are easily one of the most commonly prescribed pharmaceuticals available on the market today — even though they are known to cause a litany of harmful side effects, ranging from increasing the risk of diabetes to suicide. Newly published research has discovered a suicide biomarker in the brains of people with PTSD....
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Conclusion. This retrospective observational study with a limited statistical power did not show a different prognosis in patients with and without NOD. PMID: 31675271 [PubMed - in process]
Source: Scandinavian Cardiovascular Journal - Category: Cardiology Tags: Scand Cardiovasc J Source Type: research
More News: Chemistry | Diabetes | Endocrinology | Insulin | Nanotechnology | PET Scan