Remote sensing of Salmonella-specific DNA fragment by using nanoporous alumina modified with the single-strand DNA probe

Publication date: Available online 19 October 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Mahmoud Amouzadeh Tabrizi, Josep Ferré-Borrull, Lluis F. MarsalAbstractFoodborne Salmonella disease is a major public health concern. Therefore, it is crucial to design a reliable biosensor to the diagnosis of this disease. Herein, we fabricated an interferometric reflectance spectroscopy (IRS) based DNA sensor for the determination of Salmonella-specific DNA fragment concentration. For this purpose, the nanoporous anodic alumina (NAA) was first fabricated. The pore walls of NAA were then functionalized with 3-aminopropyl tri-methoxy silane (NAA-NH2). After that, the amino-terminated single strand DNAsal (ssDNAsal) probe was immobilized on NAA-NH2 by the use of glutaraldehyde (Glu) as cross-linker. To detect the amount of Salmonella-specific DNA fragment, the different concentrations of Salmonella-specific DNA fragment were pumped to the analytical flow cell. Subsequently, methylene blue (MB) was pumped to the analytical flow cell to intercalate between guanine-cytosine base pairs. The intercalated MB into double-strand DNA absorbed the transferred white light to the DNA sensor and therefore, the intensity of the reflected light from the DNA sensor to the charge-coupled device detector decreased. The decrease in the intensity of the reflected light consequent on the decrease in the peak area of the IRS in the wavelength range of 450-1050 nm (peak area450-1050 nm). The decr...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research