Immunohistochemical and molecular detection of natural cases of bovine rotavirus and coronavirus infection causing enteritis in dairy calves

Publication date: Available online 19 October 2019Source: Microbial PathogenesisAuthor(s): Shailendra Singh, Rajendra Singh, K.P. Singh, V. Singh, Y.P.S. Malik, Bhupesh Kamdi, Rahul Singh, Gayatri KashyapAbstractBovine rotavirus (BRoV) and bovine coronavirus (BCoV) are major enteric viral pathogens responsible for calve diarrhoea. They are widespread both in dairy and beef cattle throughout the world and causing huge economic losses. The diagnosis of these agents is very difficult due to non-specific nature of lesions and the involvement of some intrinsic and extrinsic risk factors. We performed postmortem of 45 calves, which was below three months of age. Out of 45 necropscid calves, three (6.66%) cases were positive for BRoV and four (8.88%) cases were found positive for BCoV, screened by reverse transcriptase polymerase chain reaction (RT-PCR). Further RT-PCR positive cases were confirmed by immunohistochemistry (IHC) in paraffin-embedded intestinal tissue sections. Three cases of enteritis caused by BRoV showed the hallmark lesions of the shortening and fusion of villi, denudation and infiltration of mononuclear cells in the lamina propria. The BRoV antigen distribution was prominent within the lining epithelium of the villi, peyer's patches in the ileum and strong immunoreactions in the lymphocytes and some macrophages of the mesenteric lymph nodes. Four cases in which BCoV was detected, grossly lesions characterized by colonic mucosa covered with thick, fibrinous and di...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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Publication date: Available online 20 November 2019Source: Indian Heart JournalAuthor(s): Shaimaa MostafaAbstractBackgroundFew studies have evaluated right ventricle systolic function in different categories of heart failure despite its effect on outcomes.Methods and resultssingle-centre, cross-sectional study included 150 patients, 50 patients in each category of HF: group I, preserved; group II, mid-range, group III reduced ejection fraction. Left ventricular systolic function was assessed by 3D echo, and right ventricular systolic function was assessed by fraction area change (FAC), tricuspid annular plane systolic excu...
Source: Indian Heart Journal - Category: Cardiology Source Type: research
Publication date: Available online 19 November 2019Source: Indian Heart JournalAuthor(s): Shamshad Alam, Sharad Chandra, Gaurav Chaudhary, Akhil Sharma, Monika Bhandhari, Pravesh Vishwakarma, Akshaya Pradhan, Rishi Sethi, Sudhanshu K. Dwivedi, Varun S. NarainAbstractBackgroundChemotherapy-induced cardiotoxicity constitutes subclinical myocardial dysfunction, arrhythmias, pericarditis, coronary vasospasm, significant symptomatic heart failure. Anthracyclines pose higher risk for long-term cardiac dysfunction, with increased incidences of morbidity and mortality. Hence, early detection of chemotherapy induced cardiac dysfunc...
Source: Indian Heart Journal - Category: Cardiology Source Type: research
This article provides a contemporary critique of design types, evidence base and existing limitations of this nascent technology.
Source: Indian Heart Journal - Category: Cardiology Source Type: research
Publication date: November 2019Source: Mammalian Biology, Volume 99Author(s): Sameer B. Bajaru, Amol R. Kulavmode, Ranjit ManakadanAbstractHuman-dominated ecosystems are characterized by three main processes, viz., habitat degradation, habitat loss and habitat fragmentation, these posing a great threat to biodiversity. However, the relationships between these processes are not clearly understood. Moreover, habitat loss and habitat fragmentation occur at landscape-scale and their effects depend on the spatial scale. We trapped small mammals in a human-dominated area in the northern Western Ghats, India, at 23 sites in three...
Source: Mammalian Biology - Category: Biology Source Type: research
Source: Indian Journal of Pediatrics - Category: Pediatrics Source Type: research
Publication date: Available online 19 November 2019Source: Clinical Epidemiology and Global HealthAuthor(s): Imran Kazmi, Muhammad Afzal, Muhammad Shahid Nadeem, Fahad A. Al-Abbasi, Firoz Anwar
Source: Clinical Epidemiology and Global Health - Category: Epidemiology Source Type: research
te;nai Z, Dán Á Abstract Enteric viral diseases of swine are one of the most frequent disorders causing huge economic losses in pork production. After the reappearance of an emerging enteropathogen, porcine epidemic diarrhoea virus (PEDV) in Hungary in 2016, an extensive survey was initiated in an attempt to identify diarrhoea-related porcine viruses, including adeno-, astro-, boca-, calici-, circo-, corona-, kobu-, rota- and Torque teno viruses. A total of 384 faecal samples collected during a twoyear period from diarrhoeic and asymptomatic pigs of various ages in 17 farms were screened by conventio...
Source: Acta Veterinaria Hungarica - Category: Veterinary Research Authors: Tags: Acta Vet Hung Source Type: research
Summary Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus that causes diarrhoea, vomiting and dehydration in sucking and nursing piglets. It was first reported in Hong Kong in 2012 and has since been discovered in the United States, Canada, South Korea, mainland China, Thailand and Laos. PDCoV has been experimentally proved to lead to diarrhoea in swine and it was detected positive in pigs in Guangdong, southern China. In our study, 252 faecal and intestinal samples from sucking piglets and sows with diarrhoea were surveyed for common enteric viruses. We found a prevalence of PDCoV (21.8%), porcine epidemi...
Source: Transboundary and Emerging Diseases - Category: Veterinary Research Authors: Tags: ORIGINAL ARTICLE Source Type: research
Summary A 300‐sow farrow‐to‐finish swine operation in the United States experienced a sudden and severe increase in mortality in neonatal piglets with high morbidity followed by vesicular lesions on the snout and feet of adult females and males. Affected live piglets were submitted for diagnostic investigation. Samples tested polymerase chain reaction (PCR) negative for foot‐and‐mouth disease virus, porcine delta coronavirus, porcine epidemic diarrhoea virus, porcine rotavirus types A, B and C, transmissible gastroenteritis virus, and porcine reproductive and respiratory syndrome virus. Senecavirus A (SV‐A) for...
Source: Transboundary and Emerging Diseases - Category: Veterinary Research Authors: Tags: Rapid Communication Source Type: research
Merial Animal Health has added Locatim, an oral antibody supplement to help in the management of scour in calves, to its product range. The company explains that the product is manufactured from the colostrum of high health status cows that are hyperimmunised against Escherichia coli, rotavirus and coronavirus. It says the product contains a guaranteed concentration of specific antibodies to enterotoxic E coli F5 (K99) and that, when given alongside the dam's colostrum, it provides enhanced protection against infection, reducing calf mortality due to neonatal diarrhoea caused by E coli F5 (K99), and helping to protect calf...
Source: Veterinary Record - Category: Veterinary Research Tags: Products and Services Source Type: research
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