A novel MuSK cell-based myasthenia gravis diagnostic assay
To improve the clinical diagnosis of neural autoimmune diseases, we developed an in-house muscle-specific kinase (MuSK) antibody cell-based assay (CBA) and compared its performance with RIA, ELISA, and other CBAs. Sera from patients with myasthenia gravis (MG) and other autoimmune diseases were analyzed. We found 46 (18.3%) MuSK-CBA Ab positive cases among 251 AChR-Ab negative cases [patients] and 4 (0.6%) MuSK-CBA Ab positive cases [among] the 624 AChR-Ab positive samples. Comparing these with available clinic assays, our highly specific CBA method is more sensitive than commercial ELISA and IFA(indirect immunofluorescence assay).
Source: Journal of Neuroimmunology - Category: Allergy & Immunology Authors: Jiaojiao Han, Jing Zhang, Mingqiang Li, Yingna Zhang, Jie Lv, Xue Zhao, Shumin Wang, Lulu Wang, Haonan Yang, Sensen Han, Hua Fang, Feng Gao Source Type: research