Selective recruitment of Nck and Syk contribute to distinct leukocyte immune-type receptor-initiated target interactions.

Selective recruitment of Nck and Syk contribute to distinct leukocyte immune-type receptor-initiated target interactions.
Cell Signal. 2019 Oct 15;:109443
Authors: Lillico DME, Pemberton JG, Niemand R, Stafford JL
Abstract
The ability of phagocytes to recognize, immobilize, and engulf extracellular targets are fundamental immune cell processes that allow for the destruction of a variety of microbial intruders. The phagocytic process depends on the signalling activity of immunoregulatory receptor-types that initiate dynamic changes in plasma membrane architecture required to accommodate the internalization of large particulate targets. To better understand fundamental molecular mechanisms responsible for facilitating phagocytic receptor-mediated regulation of cytoskeletal networks, our research has focused on investigating representative immunoregulatory proteins from the channel catfish (Ictalurus punctatus) leukocyte immune-type receptor family (IpLITRs). Specifically, we have shown that a specific IpLITR-type can regulate the constitutive deployment of filopodial-like structures to actively capture and secure targets to the phagocyte surface, which is followed by F-actin mediated membrane dynamics that are associated with the formation of phagocytic cup-like structures that precede target engulfment. In the present study, we use confocal imaging to examine the recruitment of mediators of the F-actin cytoskeleton during IpLITR-media...

Source: Cellular Signalling - October 14, 2019 Category: Cytology Authors: Lillico DME, Pemberton JG, Niemand R, Stafford JL Tags: Cell Signal Source Type: research