Multicenter evaluation of the new QIAstat Gastrointestinal Panel for the rapid syndromic testing of acute gastroenteritis

AbstractIn acute gastroenteritis (AGE), identification of the infectious agent is important for patient management. Since symptoms do not reliably identify the agent, microbiological diagnostics are important. Conventional methods lack sensitivity and often take days. Multiplex PCR panels offer fast and sensitive alternatives. Our aim was to assess the performance of the new QIAstat Gastrointestinal Panel (GIP) detecting 24 different gastroenteric pathogens from stool in Cary-Blair transport medium (Adenovirus F 40/41, Astrovirus, Norovirus GI/GII, Rotavirus A, Sapovirus,Campylobacter spp.,Clostridium difficile,Plesiomonas shigelloides,Salmonella spp.,Vibrio cholera,Vibrio parahaemolyticus,Vibrio vulnificus,Yersinia enterocolitica, enteroaggregativeEscherichia coli, enteropathogenicE. coli, enterotoxigenicE. coli, Shiga-toxin-producingE. coli (stx1 and stx2) (including specific detection ofE. coli O157),Shigella spp./enteroinvasiveE. coli,Cryptosporidium spp.,Cyclospora cayetanensis,Entamoeba histolytica andGiardia lamblia). We tested both prospective (n = 163) and retrospective (n = 222) stool samples sent for routine diagnostics by the QIAstat GIP comparing it to the FDA-approved BioFire FilmArray GIP. Seegene Allplex GIP was used for discrepancy testing. After discrepancy testing, QIAstat GIP detected 447 of 455 pathogens (98.2%, 95% confidence interval (CI) 96.6–99. 1%). There were eight false positive detections. Multiple pathogens were ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research

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Publication date: Available online 30 May 2020Source: American Journal of Infection ControlAuthor(s): Hiroki Kitagawa, Minako Mori, Toshinori Hara, Seiya Kashiyama, Norifumi Shigemoto, Hiroki Ohge
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
Publication date: Available online 30 May 2020Source: Pharmacology &TherapeuticsAuthor(s): Nasser Hashemi Goradel, Babak Negahdari, Sajjad Ghorghanlu, Samira Jahangiri, Arash Arashkia
Source: Pharmacology and Therapeutics - Category: Drugs & Pharmacology Source Type: research
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Source: Advances in Dermatology and Allergology - Category: Dermatology Tags: Postepy Dermatol Alergol Source Type: research
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Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Salmonella enterica subsp. enterica serovar Typhimurium Source Type: research
mente Capasso The interconversion of CO2 and HCO3− is catalyzed by a superfamily of metalloenzymes, known as carbonic anhydrases (CAs, EC 4.2.1.1), which maintain the equilibrium between dissolved inorganic CO2 and HCO3−. In the genome of Escherichia coli, a Gram-negative bacterium typically colonizing the lower intestine of warm-blooded organisms, the cyn operon gene includes the CynT gene, encoding for a β-CA, and CynS gene, encoding for the cyanase. CynT (β-CA) prevents the depletion of the cellular bicarbonate, which is further used in the reaction catalyzed by cyanase. A secon...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
This study provides insights on the possible involvement of these seven missense mutations in colorectal tumors. The hyperactive forms could lead to an increased O-fucosylation of POFUT1 protein targets such as NOTCH receptors in CRC patients, thereby leading to a NOTCH signaling dysregulation. It is the first demonstration of gain-of-function mutations for this crucial glycosyltransferase, modulating NOTCH activity, as well as that of other potential glycoproteins.
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
AbstractWe investigated the potential role of miR-490-3p in ischemia reperfusion (IR) injury. We first determined the expression of miR-490-3p and autophagy-related 4B cysteine (ATG4B) in IR. Then, to explore whether miR-490-3p would affect autophagy, apoptosis, and IR injury, we evaluated apoptosis, autophagy, and infarct size via gain- and loss-of-function experiments. Furthermore, we used adenovirus to enhance or inhibit the expression of ATG4B, and then measured autophagy, apoptosis, and IR injury. miR-490-3p was downregulated in the hearts during the process of IR, while ATG4B was upregulated. The inhibition of miR-49...
Source: Journal of Cardiovascular Translational Research - Category: Cardiology Source Type: research
Abstract Biological denitrification is a promising and green technology for air pollution control. To investigate the nitric oxide reductase (NOR) that dominates NO reduction efficiency in biological purification, the heterologous prokaryotic expression system of the norB gene, which encodes the core peptide of the catalytic reduction structure in the NOR from Achromobacter denitrificans strain TB, was constructed in Escherichia coli BL21 (DE3). Results showed that the 1218 bp-long norB gene was expressed at the highest level under 1.0 mM IPTG for 5 h at 30 °C, and the relative expression a...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
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Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
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Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
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