Characterizing the effects of thermal treatment on human norovirus GII.4 viability using propidium monoazide combined with RT-qPCR and quality assessments in mussels

This study investigated the effects of thermal treatment (70, 80, and 90 °C for 5, 10, and 20 min) on the titers of human norovirus (HuNoV) GII.4, a dominant genotype in fresh mussels using propidium monoazide (PMA) pretreatment and real-time reverse transcription quantitative-polymerase chain reaction (RT-qPCR) and the effects of thermal treatment on mussel quality (Hunter colors and sensory properties). Reductions of>1-log10 for HuNoV in PMA-treated mussels required 70 °C for 20 min, 80 °C for 10 min and 90 °C for 5 min. As assessed by PMA/RT-qPCR, the log10 reduction values of HuNoV in PMA-treated samples was significantly reduced (46% or 0.27 log10; P < 0.05) when heated to 90 °C for 20 min compared with non-PMA treated samples under identical conditions. Hunter color ‘L’-, and ‘a’- and ‘b’- values significantly (P < 0.05) decreased and increased, respectively with a stepwise increase of temperature (70–90 °C) and duration of heat treatment (5–20 min). The results of the untrained 7-point hedonic scale of sensory evaluation revealed that mussels heated to 90 °C for 10 min were the best quality sample among all thermally treated mussels. The results suggest that the PMA/RT-qPCR method could be very effective in distinguishing HuNoV viability following high heat treatment for an extended duration (eg, 90 °C for 20 min). Based on these results, the PMA/RT-qPCR method could be very effective in assessing ...
Source: Food Control - Category: Food Science Source Type: research