Bile-Salt Stimulated Lipase Polymorphisms do not Associate with HCV Susceptibility.

Bile-Salt Stimulated Lipase Polymorphisms do not Associate with HCV Susceptibility. Virus Res. 2019 Oct 14;:197715 Authors: Steba GS, Koekkoek SM, Prins M, Brinkman K, Kwa D, van der Meer JTM, van der Valk M, Molenkamp R, Pollakis G, Schinkel J, Paxton WA, MOSAIC (MSM observational Study of Acute infection with Hepatitis C) study group Abstract Bile-salt stimulate lipase (BSSL) is a glycoprotein found in human milk and blood that can potently bind DC-SIGN. The BSSL gene is highly polymorphic with a variant number of O-linked glycosylated 11 amino acid repeats at the C-terminus of the protein, encoded in exon 11 of the gene. It has been shown that certain BSSL genotypes associate with decreased HIV-1 transmission in vitro and decreased HIV-1 disease progression. The protein forms dimers and individuals possessing one high (typically 14-21) and one low (typically 7-11) number of repeat domains has been shown to have stronger binding of BSSL to DC-SIGN and HIV-1 inhibitory activity in vitro. Since we previously demonstrated that SNPs within the DC-SIGN gene can associate with risk of HCV sexual transmission and which can be linked to diminished DC-SIGN gene expression we aimed to identify whether BSSL polymorphisms associated similarly through differential binding to DC-SIGN. DNA was isolated from the HIV-1 infected MSM cohort (MOSAIC) composed of HCV multiple exposed uninfected (MEU) (N = 30) and multiple exposed HCV infected (MEI)...
Source: Virus Research - Category: Virology Authors: Tags: Virus Res Source Type: research