Development of a human scFv antibody targeting the lethal Iranian Cobra (Naja oxiana) snake venom.

Development of a human scFv antibody targeting the lethal Iranian Cobra (Naja oxiana) snake venom. Toxicon. 2019 Oct 14;: Authors: Kazemi-Lomedasht F, Yamabhai M, Sabatier JM, Behdani M, Zareinejad MR, Shahbazzadeh D Abstract Snakebite is one of the health concerns worldwide. Naja oxiana is one of the venomous snakes with a high mortality rate. Anti-serum therapy is the only treatment of the victims. However, in some cases, antiserum injection leads to some side effects in host like serum sickness and anaphylactic shock. It is crucial to develop a neutralizing agent with low side effects. The human antibody library (non-immunized library) was used to isolate specific antibodies against N.oxiana venom components. Four rounds of biopanning were performed to enrich scFv-displaying phages against the venom of N. oxiana. Enrichment of scFv-displaying phages against N. oxiana venom was analyzed by polyclonal Enzyme-Linked Immunosorbent Assay (ELISA). Specific antibody fragments against N. oxiana venom were selected through monoclonal ELISA, and were expressed in E. coli bacterial cells. Purification of the selected clones was performed by using nickel affinity chromatography. Neutralization and protective capacity of specific antibody fragments were analyzed in C57BL/6 mice (i.v. injection). Results of biopanning and polyclonal ELISA demonstrate a successful enrichment process. Five specific antibody fragments with the highest signal in mo...
Source: Toxicon - Category: Toxicology Authors: Tags: Toxicon Source Type: research