CRISPR/Cas9-Based Genome Editing of HSV.

CRISPR/Cas9-Based Genome Editing of HSV. Methods Mol Biol. 2020;2060:169-183 Authors: Velusamy T, Gowripalan A, Tscharke DC Abstract The CRISPR/Cas9 gene editing system is a robust and versatile technology that has revolutionized our capacity for genome engineering and is applicable in a wide range of organisms, including large dsDNA viruses. Here we provide an efficient methodology that can be used both for marker-based and for marker-free CRISPR/Cas9-mediated editing of the HSV-1 genome. In our method, Cas9, guide RNAs and a homology-directed repair template are provided to cells by cotransection of plasmids, followed by introduction of the HSV genome by infection. This method offers a great deal of flexibility, facilitating editing of the HSV genome that spans the range from individual nucleotide changes to large deletions and insertions. PMID: 31617178 [PubMed - in process]
Source: Herpes - Category: Infectious Diseases Authors: Tags: Methods Mol Biol Source Type: research