Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination.

Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination.
Methods Mol Biol. 2020;2060:395-407
Authors: Cooper RS, Heldwein EE
Abstract
HSV glycoproteins play important roles in the viral life cycle, particularly viral cell entry. Here we describe the protocol for expression, purification, and crystallization of full-length HSV-1 glycoprotein B. The protocol provides a framework for incorporating transmembrane domain-stabilizing amphipols into the crystallization setup and can be adapted to isolate other complete HSV glycoproteins.
PMID: 31617193 [PubMed - in process]

Source: Herpes - October 18, 2019 Category: Infectious Diseases Authors: Cooper RS, Heldwein EE Tags: Methods Mol Biol Source Type: research