A Model of In Vivo HSV-1 DNA Transport Using Murine Retinal Ganglion Cells.

We present an alternative method for studying these issues using the axonal transport of Herpes simplex virus in mature retinal neurons in vivo. Using genetically identical mice and carefully controlling the delivery of virus, an investigator can obtain insight into the transport of virus to and from the neuron cell body within the cellular environment of an intact, mature animal. This allows confirmation and extension of results seen in vitro. PMID: 31617195 [PubMed - in process]
Source: Herpes - Category: Infectious Diseases Authors: Tags: Methods Mol Biol Source Type: research

Related Links:

Abstract Background and objectives: Recurrent herpes simplex keratitis (HSK) is the most common cause of corneal blindness in the developed world. A relationship between host gene polymorphisms and the recurrence of herpes simplex virus (HSV) infection has previously been proposed. Thus, the aim of this study was to investigate a potential association between the IL28B host genotype and recurrent HSK. Materials and Methods: Eighty patients older than 18 years of age of both genders with a history of recurrent herpes simplex labialis (HSL) were considered for inclusion. Seventy-five of these patients were found to ...
Source: Medicina (Kaunas) - Category: Universities & Medical Training Authors: Tags: Medicina (Kaunas) Source Type: research
The clear tissue at the front of the eye is known as the cornea, and it must remain crystal clear in order for an individual to see clearly. The herpes viruses can infect the cornea, causing damage to tissue and possibly causing lasting visual impairment if not quickly identified and treated. Herpes simplex: the cold sore virus One of the most common infections of the cornea is caused by the herpes simplex virus (HSV type I and HSV type II), or the cold sore virus. The most common question from patients with HSV infection of the cornea is “How did I get this?” The answer is fairly simple. The virus is everywher...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Eye Health Infectious diseases Source Type: blogs
Christine I. Alston1,2 and Richard D. Dix1,2* 1Department of Biology, Viral Immunology Center, Georgia State University, Atlanta, GA, United States 2Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA, United States Suppressor of cytokine signaling (SOCS) proteins provide selective negative feedback to prevent pathogeneses caused by overstimulation of the immune system. Of the eight known SOCS proteins, SOCS1 and SOCS3 are the best studied, and systemic deletion of either gene causes early lethality in mice. Many viruses, including herpesviruses such as herpes simplex virus and cytomega...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
AbstractPeripheral nerve injury downregulates the expression of the μ-opioid receptor (MOR) and voltage-gated potassium channel subunit Kv1.2 by increasing their DNA methylation in the dorsal root ganglion (DRG). Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) causes DNA demethylation. Given that DRG MOR and Kv1.2 downregulation contribute to neuropath ic pain genesis, this study investigated the effect of DRG TET1 overexpression on neuropathic pain. Overexpression of TET1 in the DRG through microinjection of herpes simplex virus expressing full-length TET1 mRNA into the injured rat DRG significantly allevi...
Source: Neurotherapeutics - Category: Neurology Source Type: research
We describe a clinical case report in which a patient successfully maintained a sustained absence of HSV outbreaks in regions where BoNTA was intradermally administered. BoNTA may offer a novel therapeutic approach for preventing recurrent HSV disease. J Drugs Dermatol. 2018;17(10):1127-1129. PMID: 30365595 [PubMed - in process]
Source: Journal of Drugs in Dermatology - Category: Dermatology Tags: J Drugs Dermatol Source Type: research
AbstractPurpose of ReviewViruses, particularly herpes simplex virus (HSV), may be a cause of Alzheimer ’s disease (AD). The evidence supporting the viral hypothesis suggests that antiviral treatment trials, which have not been conducted, are warranted.Recent FindingsHSV1 (oral herpes) and HSV2 (genital herpes) can trigger amyloid aggregation, and their DNA is common in amyloid plaques. HSV1 reactivation is associated with tau hyperphosphorylation and possibly tau propagation. Anti-HSV drugs reduce A β and p-tau accumulation in infected mouse brains. Clinically, after the initial oral infection, herpes simplex vi...
Source: Current Neurology and Neuroscience Reports - Category: Neuroscience Source Type: research
el W. Fraser Qihan Li Herpes simplex virus type 1(HSV-1) presents a conundrum to public health worldwide because of its specific pathogenicity and clinical features. Some experimental vaccines, such as the recombinant viral glycoproteins, exhibit the viral immunogenicity of a host-specific immune response, but none of these has achieved a valid epidemiological protective efficacy in the human population. In the present study, we constructed an attenuated HSV-1 strain M3 through the partial deletion of UL7, UL41, and the latency-associated transcript (LAT) using the CRISPR/Cas9 system. The mutant strain exhibited lowe...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
ConclusionsOur results support the feasibility of using this vector for sustained neuronal expression of human frataxin for FA gene therapy.
Source: The Journal of Gene Medicine - Category: Genetics & Stem Cells Authors: Tags: RESEARCH ARTICLE Source Type: research
Women with previous herpes simplex virus type-1 (HSV-1) infection have persistent antibodies against the virus and can pass protection to their unborn babies. In laboratory studies using autopsied human tissue, the researchers showed that the antibodies remain in the trigeminal ganglion (a key site of HSV infection) in mothers and that they travel to the same site in their fetus. In studies in mice, they showed that these vertically transmitted antibodies completely protect newborn mice from infection.
Source: The Lancet Infectious Diseases - Category: Infectious Diseases Authors: Tags: Newsdesk Source Type: research
CONCLUSIONS: The detection of cytomegalovirus (CMV) and herpes simplex virus-1 (HSV-1) genome in perilymph of patients with negative serology or clinical history for congenital infections supports the hypothesis that Herpesviridae, even after acquired postnatal infections, could remain in latent phase in the spiral ganglion and damage the cochlea by a possible subsequent reactivation. Further studies are needed to identify the markers of such reactivation. OBJECTIVE: To identify the presence of certain viral species in the endolabyrinthic fluid of deaf patients with non-congenital infection. The research of viral DNA ...
Source: Acta Oto-Laryngologica - Category: ENT & OMF Authors: Tags: Acta Otolaryngol Source Type: research
More News: Biology | Brain | Cold Sores | Cytology | Environmental Health | Ganglions | Genetics | Herpes | Hormones | Infectious Diseases | Neurology | Stem Cell Therapy | Stem Cells | Study | Vitamin A