An approach to diagnosis of Jaagsiekte sheep retrovirus infection in sheep based on assessment of agreement between macroscopic examination, histopathologic examination and reverse-transcriptase polymerase chain reaction

Publication date: Available online 16 October 2019Source: Small Ruminant ResearchAuthor(s): Alison M. Lee, Alan Wolfe, Joseph P. Cassidy, John Moriarty, Ronan O’Neill, Claire Fahy, Emily Connaghan, Chris Cousens, Mark P. Dagleish, Maire C. McElroy, Locksley L. McV. MessamAbstractJaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA). JSRV infection is usually detected post-mortem by macroscopic and histological examination of lungs for lesions of OPA. Subsequently, the presence of JSRV may be confirmed using polymerase chain reaction (PCR) on tumour tissue. Our goal was to determine the most effective way of combining macroscopic and histological examination with reverse transcriptase PCR (RT-PCR) to detect JSRV infection post-mortem. Lungs of slaughtered sheep (n = 369) with macroscopic lesions were examined macroscopically and histologically to identify lesions consistent with OPA, and subsequently subjected to RT-PCR for JSRV. Positive (Ppos) and negative (Pneg) agreement and Cohen’s Kappa were calculated between RT-PCR and: 1) macroscopic examination; 2) histological examination; 3) macroscopic and histological examinations combined in series, and 4) in parallel. The highest Ppos was between macroscopic and histological examination in parallel and RT-PCR (0.38). Conversely, Pneg for all combinations of RT-PCR and macroscopic and histological examinations was high (0.95-0.96). All Kappa values were low (0...
Source: Small Ruminant Research - Category: Zoology Source Type: research

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Authors: Clemente-Tomás R, Gargallo-Benedicto A, Schilt-Catafal M, Duch-Samper AM PMID: 31740280 [PubMed - as supplied by publisher]
Source: Archivos de la Sociedad Espanola de Oftalmologia - Category: Opthalmology Tags: Arch Soc Esp Oftalmol Source Type: research
Publication date: Available online 20 November 2019Source: Inorganica Chimica ActaAuthor(s): Carlos M. Manzano, Douglas H. Nakahata, Juan C. Tenorio, Wilton R. Lustri, Flávia A. Resende Nogueira, Nadia A. Aleixo, Pietra S. da Silva Gomes, Fernando R. Pavan, Julia A. Grecco, Camila M. Ribeiro, Pedro P. CorbiAbstractThis manuscript presents three silver(I) complexes with fluoroanthranilic acid (fa) isomers, [Ag(4fa)]n, [Ag(5fa)]n and [Ag(6fa)]n, which were named as Ag4fa, Ag5fa and Ag6fa, respectively. The 1:1 metal/ligand molar composition of the complexes was determined by elemental, thermal and high-resolution mass...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
We read with great interest the article by Machicado et  al,1 which reported the results of a randomized controlled trial comparing the performance of pre-loaded fiducial needles (PLNs) versus traditional back-loaded fiducials (BLNs) in patients with pancreatic ductal adenocarcinoma (PDAC) undergoing image-guided radiation therapy (IGRT) in 2 academic t ertiary care centers. Among 44 patients enrolled into the study during a 22-month period, 22 underwent PNLs using a novel fiducial needle with 4 gold markers (PreLoad4; Cook Medical, Bloomington, IN), whereas in other 22 patients assigned to the BLN group, the Visicoil...
Source: Clinical Gastroenterology and Hepatology - Category: Gastroenterology Authors: Tags: Letter to the Editor Source Type: research
Conditions:   Gastrointestinal Tumors;   Colorectal Adenocarcinomas;   Gastric Adenocarcinomas;   Esophageal Adenocarcinomas Interventions:   Drug: PF-07062119;   Drug: Anti-PD1;   Drug: Anti-VEGF Sponsor:   Pfizer Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Future Oncology, Ahead of Print.
Source: Future Oncology - Category: Cancer & Oncology Authors: Source Type: research
CONCLUSIONS: HR-HPV RISH is highly sensitive and specific for HPV-driven endocervical glandular neoplasia compared to p16INK4a and Ki67, and should be incorporated for ECA diagnosis. PMID: 31737201 [PubMed]
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Publication date: Available online 20 November 2019Source: Materials Today CommunicationsAuthor(s): Katerina N. Panagiotaki, Konstantinos Spyrou, Michael Zachariadis, Harris Pratsinis, Antonios Kouloumpis, Lamprini G. Boutsika, Apostolos Enotiadis, Dimitrios Gournis, Emmanuel P. Giannelis, Zili SideratouAbstractA novel nanoscale drug delivery system based on monodispersed non-porous ionic silica nanospheres (NISNs) decorated with phosphonated active sides homogeneously distributed all over their surface, was developed. Doxorubicin (DOX), a well-known antitumor drug, was successfully loaded on the surface of the silica nano...
Source: Materials Today Communications - Category: Materials Science Source Type: research
Authors: Mine S PMID: 31737326 [PubMed]
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
Conclusions: The main clinicopathological type of lung cancer in young lung cancer patients aged 30 years and younger is adenocarcinoma, and most cases were at the early stage. Surgical treatment based on lobectomy is still the main treatment method and the prognosis of these patients is very good. Early screening of lung cancer should be actively promoted for young people. PMID: 31737313 [PubMed]
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
This study aimed to identify the associations of ALK rearrangement with clinicopathologic features and prognosis in patients with surgically resected stage I-IIIA lung adenocarcinoma. Methods: Analysis of ALK status was performed by a fully-automated immunochemistry assay (with rabbit monoclonal Ventana D5F3 antibody) in tissue sections of 2,103 patients with surgically-resected stage I-IIIA lung adenocarcinoma. ALK positive patients were matched with negative patients in a 1:1 ratio using propensity score matching (PSM). Clinical outcomes were assessed by disease-free survival (DFS) and overall survival (OS) after sur...
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
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