Ginsenoside Rb1 mitigates oxidative stress and apoptosis induced by methylglyoxal in SH-SY5Y cells via the PI3K/Akt pathway

Publication date: Available online 16 October 2019Source: Molecular and Cellular ProbesAuthor(s): Fengwei Nan, Guibo Sun, Weijie Xie, Tianyuan Ye, Xiao Sun, Ping Zhou, Xi Dong, Jiafu Sun, Mengren Zhang, Xiaobo SunAbstractDiabetic encephalopathy is a severe diabetic complication characterized by cognitive dysfunction and neuropsychiatric disability. Methylglyoxal (MGO), a highly reactive metabolite of hyperglycemia, serves as a major precursor of advanced glycation end products that play key roles in diabetic complications. Ginsenoside Rb1 (abbreviated as Rb1) has received extensive attention due to its potential therapeutic effects on diabetes and neurodegeneration. Therefore, this study aimed to investigate the effects of Rb1 on MGO-induced damage in SH-SY5Y cells and the related mechanism. SH-SY5Y cells were pretreated with Rb1 for 8 h and then exposed to MGO (0.5 mM) for 24 h. Cell survival was assessed by the MTT assay. Cell apoptosis was assessed using Hoechst 33342/propidium iodide (PI) staining and an Annexin-V/PI kit. The activities of oxidative stress markers were examined using commercial kits. Reactive oxygen species (ROS) staining and JC-1 staining were used to evaluate mitochondria injury. In addition, protein levels were measured by Western blot analysis. As a result, Rb1 alleviated the injury induced by MGO by increasing the activities of superoxide dismutase, catalase and total glutathione, decreasing the level of malondialdehyde, and alleviating mitocho...
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research

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Conclusions/interpretationReducing the burden of diabetes could result in substantial reductions in the incidence of dementia and disability over the medium to long term.
Source: Diabetologia - Category: Endocrinology Source Type: research
CONCLUSION: Patients with acute stroke should be treated in a specialized center whenever possible. After hospital discharge, stroke patients should be followed up at short intervals and any risk factors for stroke should be treated. PMID: 31711561 [PubMed - in process]
Source: Deutsches Arzteblatt International - Category: General Medicine Tags: Dtsch Arztebl Int Source Type: research
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Source: Proteomics - Category: Biochemistry Authors: Tags: Review Source Type: research
In this study 66 clinically mildly affected patients aged 54-87 years without a history of dementia underwent extensive neuropsychological assessment after first ever ischemic stroke and again 6 months after the event (follow-up assessment). Demographic, clinical and paraclinical parameters were assessed as potential predictors for long-term cognitive outcome. RESULTS: At the group level significant performance improvements were found for most of the neurocognitive domains at the follow-up assessment. The greatest cognitive improvement was found in visuospatial processing. Immediately after stroke 54.5% of p...
Source: Der Nervenarzt - Category: Neurology Authors: Tags: Nervenarzt Source Type: research
Authors: Giovos G, Yavropoulou MP, Yovos JG Abstract The improving effectiveness of health care leads inevitably to a rapid increase in the elderly population worldwide. At advanced ages, however, people experience chronic disabilities, which significantly increase the social and economic burden while curtailing survival, independence, and quality of life of the aging population. As aging is a multifactorial process, apart from genetic predisposition, other environmental factors, such as chronic sterile inflammation and cellular senescence, contribute as crucial participants and have been targeted to reverse their ...
Source: Hormones - Category: Endocrinology Tags: Hormones (Athens) Source Type: research
ConclusionsSome opioids provided a short ‐term substantial pain relief in highly selected patients in some neuropathic pain syndromes.
Source: European Journal of Pain - Category: Anesthesiology Authors: Tags: SYSTEMATIC REVIEW Source Type: research
Publication date: Available online 7 November 2019Source: Journal of NeuroradiologyAuthor(s): Burcu Yuksel, Pınar Koc, Eylem Ozaydin Goksu, Ertan Karacay, Fatma Kurtulus, Yesim Cekin, Yasemin Bicer GomceliABSTRACTBackground: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. We aimed to discuss possible predisposing factors to atherosclerosis such as carotid intima media thickness (CIMT) and high-sensitivity C-reactive protein (Hs-CRP) levels in MS.Methods: Thirty-five ambulatory patients with relapsing-remitting MS (RRMS) (22 females and 13 males) and 34 healthy controls (21 f...
Source: Journal of Neuroradiology - Category: Radiology Source Type: research
Publication date: Available online 7 November 2019Source: The Lancet NeurologyAuthor(s): Michael Ahmadi, Inga Laumeier, Thomas Ihl, Maureen Steinicke, Caroline Ferse, Matthias Endres, Armin Grau, Sidsel Hastrup, Holger Poppert, Frederick Palm, Martin Schoene, Christian L Seifert, Farid I Kandil, Joachim E Weber, Paul von Weitzel-Mudersbach, Martin L J Wimmer, Ale Algra, Pierre Amarenco, Jacoba P Greving, Otto BusseSummaryBackgroundPatients with recent stroke or transient ischaemic attack are at high risk for a further vascular event, possibly leading to permanent disability or death. Although evidence-based treatments for ...
Source: The Lancet Neurology - Category: Neurology Source Type: research
Purpose of review This review examines the variation in clinical practice with regards to ultrasound estimation of fetal weight, as well as calculation of fetal weight centiles. Recent findings Placental dysfunction is associated with fetal smallness from intrauterine malnutrition as well as fetal disability and even stillbirth from hypoxemia. Although estimating fetal weight can be done accurately, the issue of which fetal weight centile chart should be used continues to be a contentious topic. The arguments against local fetal growth charts based on national borders and customization for variables known to be associ...
Source: Current Opinion in Obstetrics and Gynecology - Category: OBGYN Tags: WOMEN'S HEALTH: Edited by Joseph Aquilina Source Type: research
Conclusion: This research has successfully designed and fabricated a haptic feedback device that can be used as an early warning system for diabetic automobile drivers with peripheral neuropathy.IMPLICATIONS FOR REHABILITATIONA haptic foot prototype device capable of generating warning signals to diabetic drivers whose foot could slip off the brake or accelerator pedals has been developed.The prototype includes force sensing receivers integrated with eccentric rotating mass system, a haptic controller breakout board, and Arduino software.The system is very easy to use and provides highly reliable audio and visual feedback ...
Source: Disability and Rehabilitation. Assistive Technology. - Category: Rehabilitation Authors: Tags: Disabil Rehabil Assist Technol Source Type: research
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