High glucose exposure promotes proliferation and in vivo network formation of adipose-tissue-derived microvascular fragments.
High glucose exposure promotes proliferation and in vivo network formation of adipose-tissue-derived microvascular fragments.
Eur Cell Mater. 2019 Oct 15;38:188-200
Authors: Laschke MW, Seifert MS, Scheuer C, Kontaxi E, Metzger W, Menger MD
Abstract
High glucose concentrations have been shown to activate endothelial cells and promote angiogenesis. In the present study, it was investigated whether high glucose concentrations could improve the vascularisation capacity of adipose-tissue-derived microvascular fragments (ad-MVF). Ad-MVF were isolated from the epididymal fat pads of donor mice and cultivated for 24 h in University of Wisconsin (UW) solution supplemented with vehicle or 30 mM glucose. Protein expression, morphology, viability and proliferation of the cultivated ad-MVF were analysed by means of proteome profiler mouse angiogenesis array, scanning electron microscopy and immunohistochemistry. Additional cultivated ad-MVF were seeded on to collagen-glycosaminoglycan scaffolds to study their in vivo vascularisation capacity in the dorsal skinfold chamber model by intravital fluorescence microscopy, histology and immunohistochemistry. In vitro, high glucose exposure changed the protein expression pattern of ad-MVF with endoglin, interleukin (IL)-1β and monocyte chemoattractant protein (MCP)-1 as the most up-regulated pro-angiogenic factors. Moreover, high glucose exposure induced the formation of nanopores in the ad-MVF wall. I...
Source: European Cells and Materials - Category: Cytology Tags: Eur Cell Mater Source Type: research