Exploring T & B-cell epitopes and designing multi-epitope subunit vaccine targeting integration step of HIV-1 lifecycle using immunoinformatics approach

Publication date: December 2019Source: Microbial Pathogenesis, Volume 137Author(s): Faruq Abdulla, Utpal Kumar Adhikari, M. Kamal UddinAbstractTill now, AIDS, caused by the human immunodeficiency virus (HIV) is still a severe health problem worldwide. It weakens the immune system by targeting the T-helper cells. Specifically, the severity of the pandemic HIV-1 makes the emergence of an enduring effective vaccine against HIV-1. Therefore, we have applied a series of immunoinformatics approaches to the four conserved domains of HIV-1 integrase (IN) proteins to design an effective multi-epitope based subunit vaccine which might induce a competent immunity against HIV-1. Therefore, we have selected three peptide fragments that contained all overlapping epitopes (35 CD4+, 8 CD8+ T-cell epitopes, and 3 B-cell epitopes) where the epitopes had a high conservancy score. The cumulative population coverage for combined CD8+ and CD4+ T-cell epitopes and their respective HLA-alleles were found as 98.03% in the world which is also followed by East Asia (96.24%), South Asia (96.31%), North Africa (96.14%), North America (98.99%), and Europe (98.80%). The proposed vaccine composed by an adjuvant (β-defensin) at the N-terminal site of the vaccine constructs and three peptide fragments where the adjuvant was fused by EAAAK linker and the peptide fragments were fused by GPGPG linker. The designed final vaccine construct (length: 159 amino acid) was found to be antigenic and non-allergic,...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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The evidence shows that people with inflammatory bowel disease are at increased risk of herpes zoster.1 The cohort study conducted by Khan et  al2 published in Clinical Gastroenterology and Hepatology revealed that vaccination for herpes zoster was associated with a decreased risk of herpes zoster among older people with inflammatory bowel disease. Khan et al found that the incidence of herpes zoster was higher for unvaccinated than vac cinated people (6.97 versus 4.09 per 1000 patient-years).2 The absolute risk reduction was about 0.288%, resulting in the number needed to treat of 347.
Source: Clinical Gastroenterology and Hepatology - Category: Gastroenterology Authors: Tags: Letter to the Editor Source Type: research
Condition:   Middle East Respiratory Syndrome Coronavirus Intervention:   Biological: ChAdOx1 MERS Sponsors:   King Abdullah International Medical Research Center;   University of Oxford Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
The quilt ’s more than 50,000 panels will move to the National AIDS Memorial, while its archive of personal items will go to the Library of Congress.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Quilts Culture (Arts) Acquired Immune Deficiency Syndrome Archives and Records AIDS Memorial Quilt National AIDS Memorial American Folklife Center Library of Congress the NAMES Project Foundation Golden Gate Park (San Francisco) Source Type: news
This NIHR Signal says that the risk of shingles is about 10% per year in adults after autologous stem cell transplant. The evidence summary indicates that two doses of deactivated herpes zoster vaccine could be a safe and effective way to reduce that risk by about two thirds.This effect is similar to another, heat-treated, non-live vaccine. Either vaccine could reduce the pain and need for medical treatment associated with shingles, and long-lasting complications such as post-herpetic neuralgia. It's not yet known if people need to continue taking acyclovir as well.
Source: Current Awareness Service for Health (CASH) - Category: Consumer Health News Source Type: news
[Cameroon Tribune] This was during the 11th Ordinary Session of the Scientific Council of the Research Centre yesterday, November 19, 2019 in Yaounde.
Source: AllAfrica News: HIV-Aids and STDs - Category: Infectious Diseases Source Type: news
Officials have restarted the distribution of a Flu vaccine for one million children following “concerning delays”.
Source: HSJ - Category: UK Health Source Type: news
CONCLUSION: Our findings revealed that Influence of colonic dialysis using Chinese medicine on creatinine decomposition by intestinal bacteria in uremia rats. PMID: 31737209 [PubMed]
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Publication date: 19 November 2019Source: Cell Reports, Volume 29, Issue 8Author(s): Wiebke Hartmann, Marie-Luise Brunn, Nadine Stetter, Nicola Gagliani, Franziska Muscate, Stephanie Stanelle-Bertram, Gülsah Gabriel, Minka BreloerSummaryHelminth parasites infect more than a quarter of the human population and inflict significant changes to the immunological status of their hosts. Here, we analyze the impact of helminth infections on the efficacy of vaccinations using Litomosoides sigmodontis-infected mice. Concurrent helminth infection reduces the quantity and quality of antibody responses to vaccination against seaso...
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: 19 November 2019Source: Cell Reports, Volume 29, Issue 8Author(s): Ying Xu, Ruchao Peng, Wei Zhang, Jianxun Qi, Hao Song, Sheng Liu, Haiyuan Wang, Min Wang, Haixia Xiao, Lifeng Fu, Zheng Fan, Yuhai Bi, Jinghua Yan, Yi Shi, George F. GaoSummarySince 2013, H7N9 avian influenza viruses (AIVs) have caused more than 1,600 human infections, posing a threat to public health. An emerging concern is whether H7N9 AIVs will cause pandemics among humans. Molecular analysis of hemagglutinin (HA), which is a critical determinant of interspecies transmission, shows that the current H7N9 AIVs are still dual-receptor trop...
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: 19 November 2019Source: Cell Reports, Volume 29, Issue 8Author(s): Chulwoo Kim, Rohit R. Jadhav, Claire E. Gustafson, Megan J. Smithey, Alec J. Hirsch, Jennifer L. Uhrlaub, William H. Hildebrand, Janko Nikolich-Žugich, Cornelia M. Weyand, Jörg J. GoronzySummaryGeneration of protective immunity to infections and vaccinations declines with age. Studies in healthy individuals have implicated reduced miR-181a expression in T cells as contributing to this defect. To understand the impact of miR-181a expression on antiviral responses, we examined LCMV infection in mice with miR-181ab1-deficient T&nbs...
Source: Cell Reports - Category: Cytology Source Type: research
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