Reserpine attenuates biofilm formation and virulence of Staphylococcus aureus

This study investigated the effects of reserpine, the main bioactive compound of Rauwolfia serpentina, on biofilm formation and biofilm-associated virulence factors production in a Gram-positive pathogen, Staphylococcus aureus. Crystal violet assay, MTT assay, Congo red binding, CLSM studies were performed to assess the antibiofilm activity. Molecular docking was performed to explain the possible mode of action, catheter model was used to evaluate its application potential and the combinatorial study was performed in search of an improved therapeutic formulation. Reserpine affected biofilm formation, EPS production, biofilm cell viability and virulence factor production. It could eradicate 72.7% biofilm at ½ × MIC dose and could also stop the metabolic activity of 50.6% bacterial cells in a biofilm. Staphylococcus aureus biofilm- and virulence-regulatory proteins like AgrA, AtlE, Bap, IcaA, SarA and SasG were found to interact with reserpine which might lead to the attenuation of its pathogenicity. Reserpine along with other commercial antibiotics could generate a hightened antibiofilm response, and also eradicated a good percentage of bacterial biofilm from a urinary catheter model. These findings suggested reserpine as a good alternative entity to generate new improved therapeutic formulations.Graphical abstract
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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Conclusion. The hexane extract of L. apiculata leaves showed the lower MIC against S. aureus when compared with extracts obtained from other parts of the plant. The growth curve and the colonies count suggest a bacteriostatic activity of the L. apiculata leaves extract against Staphylococcus aureus. PMID: 31737073 [PubMed]
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
Publication date: Available online 18 November 2019Source: Innovative Food Science &Emerging TechnologiesAuthor(s): Oscar G. Martínez-Moreno, Luis M. Anaya-Esparza, Jorge A. Sánchez-Burgos, Libier Meza-Espinoza, Alejandro Pérez-Larios, J. Emanuel Bojorquez-Quintal, Efigenia Montalvo-GonzálezAbstractThe combined effect of vacuum, heat and ultrasound (vacuum-thermosonication, VTS) on soursop puree was investigated with regard to the viability of Escherichia coli and Staphylococcus aureus, inactivation of polyphenol oxidase and sensory quality. The VTS conditions were: vacuum (8.46, 11 and 16.9...
Source: Innovative Food Science and Emerging Technologies - Category: Food Science Source Type: research
The association between Salmonella spp. and osteoarticular infections in pediatric patients with major sickle hemoglobinopathies has been well established. However, the contemporary microbiology of these infections in such patients is unknown. We conducted a retrospective review of medical records at Texas Children’s Hospital in Houston from 2000 to 2018 to investigate this question. Fifty cases were identified. In 23 (46%) cases, a pathogen was identified. Salmonella was the most common pathogen isolated, accounting for 61% of culture-positive cases followed by Staphylococcus aureus (21.7%).
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Brief Reports Source Type: research
During my nine years in the NFL, I ruptured tendons in my fingers, suffered multiple concussions, and broke several bones. I tore my ACL, MCL, and PCL, and completely blew out my left knee. That last injury should have ended my career. But it didn’t. I put myself back together and played through the pain. What finally took me down? A tiny bug I had never heard of and couldn’t see. A drug-resistant staph infection called methicillin-resistant Staphylococcus aureus cost me my NFL career—and almost my life. MRSA is one of many deadly, antibiotic-resistant infections. Together, these “superbugs” k...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized health Source Type: news
Abstract During the course of screening for new metabolites from basidiomycetes, we isolated and characterized five previously undescribed secondary metabolites, skeletocutins M–Q (1–5), along with the known metabolite tyromycin A (6) from the fruiting bodies of the polypore Skeletocutis sp. The new compounds did not exhibit any antimicrobial, cytotoxic, or nematicidal activities. However, compound 3 moderately inhibited the biofilm formation of Staphylococcus aureus (S. aureus), while compounds 3 and 4 performed moderately in the ʟ-leucine-7-amido-4-methylcoumarin (ʟ-Leu-AMC) inhibition assay. These compound...
Source: Beilstein Journal of Organic Chemistry - Category: Chemistry Authors: Tags: basidiomycete polyporaceae secondary metabolites structure elucidation Full Research Paper Source Type: research
In this study, the obtained IC50 concentration of 51.25 μg/mL exhibits significant anticancer activity against A549 cells than other tested concentrations because of the release of zinc ions, which induce the cell apoptosis. Furthermore, studies on cell cycle validate that ZnO NPs arrest the cell cycle at the sub-G1 phase of A549 cells. These results suggest the utility of phytogenic ZnO NPs for various biomedical applications.Graphical abstract
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
The aim of this study was to determine the antimicrobial efficiency of piperitenone epoxide (PEO) – a principal component of various aromatic plants’ essential oil – in combination with various antibiotics against 28 strains of Staphylococcus aureus and 10 strains of Escherichia coli isolated from clinical samples. Mentha spicata’s essential oil, initially collected by hydrodistillation, was then subjected to flush column chromatography affording PEO of high purity. Minimum inhibitory concentrations of PEO alone and in combination with various concentrations of antibiotics were assessed using the mi...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Staphylococcus aureus asymptomatically colonizes a third of the world ’s population, and it is an opportunistic pathogen that can cause life threatening diseases. To diagnose S. aureus infections, it is necessary to differentiate S. aureus from the ubiquitous human commensal Staphylococcus epidermidis , which beneficially colonizes the skin of all humans. Efforts are underway to identify volatile biomarkers for diagnosing S. aureus infections, but to date no studies have investigated whether S. aureus and S. epidermidis can be reliably differentiated under a variety of growth conditions. The overall goal of thi...
Source: Journal of Breath Research - Category: Respiratory Medicine Authors: Source Type: research
Dhiviya Prabaa Muthuirulandi Sethuvel, Naveen Kumar Devanga Ragupathi, Yamuna Devi Bakthavatchalam, Saranya Vijayakumar, Rosemol Varghese, Chaitra Shankar, Jobin John Jacob, Karthick Vasudevan, Divyaa Elangovan, Veeraraghavan BalajiIndian Journal of Medical Microbiology 2019 37(2):147-162 The prime goal of molecular epidemiology is to identify the origin and evolution of pathogens, which can potentially influence the public health worldwide. Traditional methods provide limited information which is not sufficient for outbreak investigation and studying transmission dynamics. The recent advancement of next-generation sequen...
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research
Conclusion: Further studies on understanding the role of the identified molecular targets such as SAA6008_00181, SAA6008_01127, agrA, agrC and coa in adapting to the pressure of antibiotics at sub-inhibitory concentrations can help in learning the molecular mechanisms causing resistance to the pathogens as well as finding other potential therapeutics.
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research
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