Reduction of turnaround time for non-tuberculous mycobacteria detection in heater-cooler units by propidium monoazide real-time PCR

Publication date: Available online 16 October 2019Source: Journal of Hospital InfectionAuthor(s): Savina Ditommaso, Monica Giacomuzzi, Gabriele Memoli, Rossana Cavallo, Antonio Curtoni, Maria Avolio, Carlo Silvestre, Carla M. ZottiSummaryBackgroundInvasive non-tuberculous mycobacteria (NTM) infections are emerging worldwide in patients undergoing open-chest cardiac bypass surgery exposed to contaminated heater-cooler units (HCUs). Although this outbreak has been investigated by culturing bacteria isolated from HCU aerosol and water samples, these conventional methods have low-analytic sensitivity, high rates of sample contamination and long turnaround time (TAT).AimTo develop a simple and effective method to detect NTM in HCUs by real-time PCR, with a short laboratory TAT and reliable culture results.MethodsA total of 281 water samples collected from various HCUs at 7 Italian hospitals were simultaneously screened for NTM by a propidium monoazide (PMA)-PCR assay and by conventional culture testing. The results were analyzed with culture testing being the reference method.Findingsi) The agreement between culture testing and PMA-PCR was 85.0% with a Ct cut-off value of
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research

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