Mycobacterium bovis prevalence affects the performance of a commercial serological assay for bovine tuberculosis in African buffaloes

This study determined the BTB prevalence in free-ranging buffaloes in two game reserves and assessed the influence of the prevalence of mycobacterial infections on the performance of a commercial cattle-specific serological assay for BTB (TB ELISA). Buffaloes (n = 997) were tested with the tuberculin skin test and TB ELISA; a subset (n = 119) was tested longitudinally. Culture, PCR and sequencing were used to confirm infection with M. bovis and/or non-tuberculous mycobacteria (NTM). Prevalence of BTB, but not NTM, influenced the TB ELISA performance. Multiple testing did not increase test confidence. The findings strongly illustrate the need for development of novel assays that can supplement existing assays for a more comprehensive testing scheme for BTB in African buffaloes.
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research

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To report on the prevalence and clinical implications of positive QuantiFERON-Gold (QFT-G) test results in the diagnostic work-up of a large cohort of consecutive patients with uveitis in the Netherlands.
Source: American Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Original Articles Source Type: research
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Source: Israel Journal of Health Policy Research - Category: International Medicine & Public Health Source Type: research
Authors: Çulha G, Kaya T, Gülbol Duran G, Urhan Küçük M, Doğramacı AÇ, Tiyekli Çelik D Abstract Leishmaniasis is a parasitic disease that is transmitted to humans by the bites of infected female phlebotomine sandflies. In the diagnosis of cutaneous leishmaniasis (CL), in the smear samples, the demonstration of the parasite by microscope remains a gold standard method. However, it becomes difficult to diagnose the parasite since the number of amastigotes in chronic cases with a lesion of one year or longer is very low. Due to many factor such as patients primarily do not ...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
In conclusion, T-SPOT.TB was found more reliable in the diagnosis of LTBI in HIV-infected individuals. In the light of these findings, especially in HIV-infected patients with low CD4+ T lymphocyte counts, T-SPOT.TB test can be considered for LTBI diagnosis. PMID: 31709936 [PubMed - in process]
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
In this study, we evaluated cellular and humoral immune responses induced by our newly designed multi-epitope vaccine in BALB/c mice. Four antigenic proteins, including histone H1, sterol 24-c-methyltransferase (SMT),Leishmania-specific hypothetical protein (LiHy), andLeishmania-specific antigenic protein (LSAP) were chosen for the prediction of potential immunodominant epitopes. Moreover, to enhance vaccine immunogenicity, two toll-like receptors 4  (TLR4) agonists, resuscitation-promoting factors ofMycobacterium tuberculosis (RpfE and RpfB), were employed as the built-in adjuvants. Immunization with the designed mul...
Source: Medical Microbiology and Immunology - Category: Microbiology Source Type: research
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Source: Microbes and Infection - Category: Infectious Diseases Source Type: research
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Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
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Source: Revista Espanola de Quimioterapia - Category: Drugs & Pharmacology Authors: Tags: Rev Esp Quimioter Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Clinical Case Reports - Category: General Medicine Authors: Tags: CASE REPORT Source Type: research
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