Selective and sensitive fluorescence "turn-on" detection of 4-thiouridine in nucleic acids via oxidative amination.

Selective and sensitive fluorescence "turn-on" detection of 4-thiouridine in nucleic acids via oxidative amination.
Chem Commun (Camb). 2019 Oct 15;:
Authors: Wang J, Shang J, Qin Z, Tong A, Xiang Y
Abstract
A fluorescence "turn-on" method for digestion-free analysis of 4-thiouridine (s4U) in nucleic acids was developed in this work based on the oxidative amination of s4U by fluoresceinamine (FAM-NH2) and periodate (IO4-). It was 125-fold more sensitive for s4U detection than the traditional UV330 absorption method, and showed excellent selectivity to s4U over 2-thiouridine (s2U) analogues and biological thiols.
PMID: 31612162 [PubMed - as supplied by publisher]

Source: Chemical Communications - October 14, 2019 Category: Chemistry Authors: Wang J, Shang J, Qin Z, Tong A, Xiang Y Tags: Chem Commun (Camb) Source Type: research