Characterization of a native Bacillus velezensis-like strain for the potential biocontrol of tropical fruit pathogens

Publication date: Available online 16 October 2019Source: Biological ControlAuthor(s): Manuela Reyes-Estebanez, Patricia Sanmartín, Juan Carlos Camacho-Chab, Susana C. De la Rosa-García, Manuel Jesús Chan Bacab, Ruth Noemí Águila Ramírez, Francisco Carrillo-Villanueva, Erika De la Rosa-Escalante, Jorge Luis Arteaga-Garma, Miguel Serrano, Benjamín Otto Ortega-MoralesAbstractPostharvest fungal decay causes economic loss and may cause human intoxication. Biological control of fungal decay of fruits using antagonistic native bacteria is a promising alternative. Leaf-associated (phyllosphere) bacteria are a promising yet underexploited source of antagonists for fungal control. A total of 108 bacterial isolates from mango (Mangifera indica L.) leaves from orchards in the Campeche state (southern Mexico) were isolated on PDA medium and screened to determine their in vitro antifungal activity against Colletotrichum gloeosporioides var. minor ATTC 42374. Selected antagonistic isolates TS3B-44, TS3B-45, TS3B-92 and TS3B-183 were subject to a comparative analysis to determine their in vitro control potential. The most effective isolate was TS3B-45, identified by 16S rDNA sequencing as being more closely related to Bacillus velezensis; its effect was compared with different control methods: chitosan, two synthetic fungicides (benomyl and prochloraz), NaHCO3 and Bacillus subtilis ATCC 55614. TS3B-45 isolate showed inhibitory activity in vitro (80%), similar to prochloraz (82%) and ...
Source: Biological Control - Category: Biology Source Type: research