Off-on fluorogenic substrate harnessing ESIPT and AIE features for in situ and long-term tracking of β-glucuronidase in Escherichia coli

Publication date: Available online 14 October 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Xianhu Wei, Qingping Wu, Ying Feng, Minling Chen, Shuhong Zhang, Moutong Chen, Jumei Zhang, Guangzhu Yang, Yu Ding, Xiaojuan Yang, Qinghua Ye, Youxiong Zhang, Qihui Gu, Juan Wang, Shi Wu, Rui Pang, Ying LiAbstractDetection and isolation of both O157:H7 and non-O157:H7 Escherichia coli are essential for the evaluation and surveillance of the associated foodborne disease risk. However, the development of a chromogenic/fluorogenic culture medium for detection and isolation is challenging yet urgent. β-Glucuronidase (GUS) serves as an important biomarker in E. coli. A novel and simple off-on fluorogenic substrate (BTBP-Gluc) was developed, based on a solid-state fluorophore (BTBP) with excited-state intramolecular proton transfer (ESIPT) and aggregation-induced emission (AIE) effects. BTBP-Gluc could be well-suited for visually detecting GUS activity under acidic conditions (3.0 ≤ pH＜7.0). It was successfully applied for detection of endogenous intracellular GUS activity in living E. coli and could produce the in situ and long-term localization of the target colonies and cell imaging of living E. coli. Additionally, it showed high selectivity, little toxicity, excellent cell permeability and sensitivity. BTBP-Gluc was combined with two tightly localizing chromogenic substrates, Magenta-Gal and X-Glu. These combinations were applied to the development of a new chromogenic-flu...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research