Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity [Biochemistry]

RNA-guided CRISPR-Cas9 proteins have been widely used for genome editing, but their off-target activities limit broad application. The minimal Cas9 ortholog from Staphylococcus aureus (SaCas9) is commonly used for in vivo genome editing; however, no variant conferring high genome-wide specificity is available. Here, we report rationally engineered SaCas9 variants with...

Source: Proceedings of the National Academy of Sciences - October 14, 2019 Category: Science Authors: Yuanyan Tan, Athena H. Y. Chu, Siyu Bao, Duc Anh Hoang, Firaol Tamiru Kebede, Wenjun Xiong, Mingfang Ji, Jiahai Shi, Zongli Zheng Tags: PNAS Plus Source Type: research