MiR-126 targets IL-17A to enhance proliferation and inhibit apoptosis in high-glucose-induced human retinal endothelial cells.

MiR-126 targets IL-17A to enhance proliferation and inhibit apoptosis in high-glucose-induced human retinal endothelial cells. Biochem Cell Biol. 2019 Oct 13;: Authors: Chen X, Yu X, Li X, Li L, Li F, Guo T, Guan C, Miao L, Cao G Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus (DM), which results in vision loss. The present study aimed to explore the role of miR-126 in high-glucose-induced human retinal endothelial cells (HRECs) and its underlying molecular mechanism. The results showed that expression of miR-126 and interleukin-17A (IL-17A) in high-glucose-induced HRECs was downregulated and upregulated, respectively. Functionally, overexpression of miR-126 promoted proliferation and suppressed apoptosis in high-glucose-induced HRECs, while IL-17A reversed the effects induced by miR-126. However, overexpression of IL-17A inhibited the proliferation and induced apoptosis, while knockdown of IL-17A accelerated the proliferation and repressed apoptosis. In addition, miR-126 repressed the expression of IL-17A, Bax, and caspase-3,while promotingthe expression of survivin and phosphorylation of PI3K and AKT;restoration of IL-17A rescued these effects. Furthermore, IL-17A was identified as a target of miR-126. Altogether, miR-126 enhances proliferation and inhibits apoptosis in high-glucose-induced HRECs by activating the PI3K/AKT pathway, increasing survivinand decreasing Bax and caspase-3 expression by ta...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research