Detection of Intracellular Cytokines

The intracellular cytokine method allows for a multiparametric readout by flow cytometry with precise phenotyping of the responding T cells. The intracellular cytokine staining of cells that have been fixed and permeabilized following a short-term activation and staining with antibodies to cell surface markers allows for identification of the cellular origin of the cytokine accumulated product.
Source: Springer protocols feed by Immunology - Category: Allergy & Immunology Source Type: news