MicroRNA gga-miR-455-5p Suppresses Newcastle Disease Virus Replication via Targeting Cellular Suppressors of Cytokine Signaling 3

In this study, we showed that expression of the cellular miRNA gga-miR-455-5p was downregulated in vivo and in vitro in response to NDV infection. Next, we found that the transfection of chicken embryonic fibroblasts (CEFs) with gga-miR-455-5p suppressed NDV replication, while the blockade of endogenous gga-miR-455-5p expression with inhibitors enhanced NDV replication. In addition, gga-miR-455-5p enhanced the expression of type I interferon and the interferon-inducible genes (ISGs) OASL and Mx1 by targeting SOCS3, a negative regulator of type I IFN signaling. Altogether, these findings highlight the crucial role of gga-miR-455-5p in host defense against NDV by targeting the SOCS3 gene to inhibit NDV replication.
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research

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Publication date: 19 November 2019Source: Cell Reports, Volume 29, Issue 8Author(s): Ying Xu, Ruchao Peng, Wei Zhang, Jianxun Qi, Hao Song, Sheng Liu, Haiyuan Wang, Min Wang, Haixia Xiao, Lifeng Fu, Zheng Fan, Yuhai Bi, Jinghua Yan, Yi Shi, George F. GaoSummarySince 2013, H7N9 avian influenza viruses (AIVs) have caused more than 1,600 human infections, posing a threat to public health. An emerging concern is whether H7N9 AIVs will cause pandemics among humans. Molecular analysis of hemagglutinin (HA), which is a critical determinant of interspecies transmission, shows that the current H7N9 AIVs are still dual-receptor trop...
Source: Cell Reports - Category: Cytology Source Type: research
This study highlights the remarkable genetic diversity in Blastocystis sp. isolates from zoo animals and provides the first molecular evidence from spotted deer, gayal and grey langur. Due to circulation of large percentage of potentially zoonotic subtypes in the wild animals, there is a higher risk of zoonotic transmission of Blastocystis sp. in the zoo keepers and visitors.Graphical abstract
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
In this study, we demonstrate a simple and rapid strategy for visual detection of multiple strains of IAVs (H1 to H16 subtypes) using peptide nucleic acid (PNA) as a biosensor and unmodified gold nanoparticles (AuNPs) as a reporter. The design principle of the assay is based on the color change on account of free PNA-induced aggregation of AuNPs in the presence of non-complementary viral RNA sequence and vice-versa. The assay could detect IAV RNA with a visual limit of detection of 2.3 ng. The quantification of RNA with a considerable accuracy on a simple spectrophotometer was achieved on plotting the PNA-induced colorim...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
Global environmental change is leading to an increase in the frequency, intensity, and duration of extreme weather events, so effective environmental management requires an understanding not only of the physiological response of organisms to increased mean temperatures, but also to extreme environmental conditions. To determine the physiological consequences of heatwaves on energy and water balance of arid-adapted zebra finches (Taeniopygia guttata), we measured field metabolic rate and water turnover rate of wild, free-living finches during a heatwave (consecutive days of maximum ambient temperature of 40–45°C) ...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
Publication date: Available online 18 November 2019Source: Comparative Biochemistry and Physiology Part A: Molecular &Integrative PhysiologyAuthor(s): L. Gerardo Herrera M, Beatriz F. Esnard H, L. Margarita Sánchez, Carlos A. Mancina GAbstractNectarivorous vertebrates might include sugar-dilute nectar in their diet and they are expected to undergo compensatory feeding. However, physiological constraints might limit the intake of sugar-dilute nectar, affecting energy budgets. Among other physiological processes, the limiting role of osmoregulation is supported by enhanced intake rate of dilute sugar solutions by ...
Source: Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology - Category: Biochemistry Source Type: research
This study demonstrates the usefulness of the HPAI H5N1 HA2 E29 epitope as a DIVA antigen in HPAI H5N1 vaccinated and infected chickens.Research Highlight E29 (HA2 position 488-516) epitope is antigenic in chickenAntibodies to E29 are elicited following live H5N1 virus infection in chickenE29 epitope is a potential DIVA antigen for use in ELISA. PMID: 31738584 [PubMed - as supplied by publisher]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
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Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
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Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
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Source: Molecular Phylogenetics and Evolution - Category: Molecular Biology Source Type: research
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