Encompassment of isoeugenol in 2-hydroxypropyl-β-cyclodextrin using ultrasonication: Characterization, antioxidant and antibacterial activities

Publication date: 15 December 2019Source: Journal of Molecular Liquids, Volume 296Author(s): Subramanian Siva, Changzhu Li, Haiying Cui, Lin LinAbstractIsoeugenol is a natural dual antioxidant/prooxidant. In this research, the inclusion complex (IC) of isoeugenol with 2-hydroxypropyl-β-cyclodextrin (HPβCD) was prepared via the ultrasound (US) method. The US assisted isoeugenol/HPβCD-IC was investigated by various characterization techniques such as UV–Vis absorption, fluorescence, powder X-ray diffraction, Fourier transform infrared spectroscopy, thermal gravimetric analysis and differential scanning calorimetry. Absorption, fluorescence and infrared studies indicated that the alkyl chain of isoeugenol was deeply included in the HPβCD cavity. Through determinations of phase solubility and water solubility studies, significant enhancement of isoeugenol water solubility was confirmed after IC formation with the HPβCD. Thermal data proved that the IC formation significantly enhanced the thermal stability of isoeugenol. Antioxidant test results indicated that isoeugenol/HPβCD-IC exhibited better antioxidant activity than free isoeugenol due to its solubility enhancement. Furthermore, the isoeugenol/HPβCD-IC showed higher antibacterial activity of 96 ± 0.2% and 97 ± 0.5% against Staphylococcus aureus and Escherichia coli bacteria, respectively.Graphical abstract
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research