Glucosinolate Metabolites in Human Urine and Plasma After Broccoli Consumption Using Non-targeted and Targeted Metabolomic Analyses

Publication date: Available online 14 October 2019Source: Food ChemistryAuthor(s): Jianghao Sun, Craig S. Charron, Janet A. Novotny, Bing Peng, Liangli Yu, Pei ChenAbstractBroccoli is a popular brassica vegetable and its consumption may decrease the occurrence of cancer in certain populations. To gain insight into the metabolites that may induce physiological responses to broccoli intake, a non-targeted metabolomic approach and a targeted approach for analysis of glucosinolate metabolites were developed using high resolution accurate mass spectrometry. A human study was conducted in which 6 subjects consumed a single meal of 200 g of uncooked broccoli florets. The metabolomic analysis revealed changes in endogenous metabolites and a decrease in hippuric acid after broccoli consumption. Targeted analysis using high-resolution, accurate mass-mass spectrometry (HRAM-MS) enabled detection of low concentrations (nM) of glucosinolate metabolites in human urine and plasma. Glucosinolate metabolites were found in human urine (13) and plasma (8), respectively. Metabolites from methoxyl-indole glucosinolates, arising from broccoli consumption, are reported for the first time. Most glucosinolate metabolites reached their peak concentration in urine 2-4 h after consumption while, in plasma, peak maxima were achieved 2 h after intake. The results suggest that glucoraphanin metabolites (sulforaphane, sulforaphane cysteine, sulforaphane N-acetyl cysteine) and indole metabolites (ascorbigen ...
Source: Food Chemistry - Category: Food Science Source Type: research

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Source: European Urology - Category: Urology & Nephrology Source Type: research
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Source: European Urology - Category: Urology & Nephrology Source Type: research
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Source: European Urology - Category: Urology & Nephrology Source Type: research
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Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Individuals who have multiple close relatives with pancreatic cancer should undergo surveillance for pancreatic cancer, according to updated recommendations from the International Cancer of the Pancreas Screening (CAPS) Consortium.Reuters Health Information
Source: Medscape General Surgery Headlines - Category: Surgery Tags: Hematology-Oncology News Source Type: news
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Source: Pharmacological Research - Category: Drugs & Pharmacology Source Type: research
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Source: Journal of Evidence Based Dental Practice - Category: Dentistry Source Type: research
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Source: Journal of Evidence Based Dental Practice - Category: Dentistry Source Type: research
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Source: Acta Pharmaceutica Sinica B - Category: Cancer & Oncology Source Type: research
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