Streptococcal peptides that signal Enterococcus faecalis cells carrying the pheromone ‐responsive conjugative plasmid pAM373

AbstractPheromone ‐mediated conjugative transfer of enterococcal plasmids can contribute to the dissemination of genes involved in antibiotic resistance, fitness, and virulence among co‐residents of mixed microbial communities. We have previously shown that intergeneric signaling by theStreptococcus gordonii strain Challis heptapeptide s.g.cAM373 (SVFILAA) induces an aggregation ‐substance‐mediated mating response and facilitates plasmid transfer fromEnterococcus faecalis cells carrying the pheromone ‐responsive plasmid pAM373 to both pheromone producing and non‐pheromone producing oral streptococcal recipients. To further investigate the streptococcal pheromone‐like peptides s.g.cAM373‐like sequences were identified in the signal sequences of streptococcal CamG lipoproteins and their ab ilities to induce a mating response inE. faecalis/pAM373 cells were examined. Synthetic heptamers with the consensus sequence (A/S) ‐(I/V)‐F‐I‐L‐(A/V/T)‐(S/A) induced AS‐mediated clumping. The conserved pheromone ABC transporter encoded byS. gordonii genome loci SGO_RS02660 and SGO_RS02665 was identified and confirmed to be required for s.g.cAM373 activity. Functional assays of cultures supernatants from representative oral and blood isolates ofS. gordonii showed that in addition to strains encoding s.g.cAM373, strain SK120, encoding the newly ‐identified pheromone s.g.cAM373‐V (SVFILVA), was able to induce enterococcal clumping, whereas strains SK6, SK8, SK9...
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research

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ConclusionsAn antibiotic allergy label was associated with significantly higher rates of MRSA colonization but no statistical difference with VRE colonization.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
Abstract Safety aspects and probiotic properties of Enterococcus faecium FL31 strain producing an enterocin, named BacFL31 were previously demonstrated. Taking into account its originality, the enterocin BacFL31 was added alone at 200 AU/g or in combination with the aqueous peel onion (Allium cepa) extract (APOE) at 1.56 ± 0.3 mg/mL to ground beef meat. Its biopreservative effect was evaluated by microbiological, physicochemical and sensory analyses during 14 days at 4°C. The APOE was characterized for its phytochemical content: total phenolic (TPC), flavonoids (TFC) and tannins contents (TAC), its anti...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
ConclusionsBased on the developed reference dataset, clinically relevant streptococcal and enterococcal species can now be reliably identified by 16S –23S rRNA sequences in samples. This study will be useful for introduction of a novel diagnostic tool, NGS of the 16S–23S rRNA region, which undoubtedly is an improvement for reliable culture-independent species identification directly from polymicrobially constituted clinical samples.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Abstract The actinomycete strain FIM06-0036 was isolated from marine sponge sample collected from the East China Sea and identified as Verrucosispora sp. based upon the results of 16S rRNA sequence analysis. One new alkaloid, 2-ethylhexyl 1H-imidazole-4-carboxylate (1), together with a known alkaloid butyl 1H-imidazole-4-carboxylate (2) was obtained from the fermentation products of this strain, the structures of compounds 1 and 2 were determined by their detailed analysis of 1 D, 2 D NMR and HR-ESI-MS data, along with literature data analysis. Compounds 1 and 2 were evaluated for their antimicrobial...
Source: Natural Product Research - Category: Biochemistry Authors: Tags: Nat Prod Res Source Type: research
Publication date: Available online 8 November 2019Source: Food Research InternationalAuthor(s): Marco Túlio Pardini Gontijo, Jackson de Sousa Silva, Pedro Marcus Pereira Vidigal, José Guilherme Prado MartinAbstractThe microbiota contributes to artisanal cheese bioprotection and biopreservation through inter and intraspecific competition. This work aimed to investigate the phylogenetic distribution of the repertoire of bacteriocin structural genes of model lactic acid bacteria (LAB) in order to investigate its respective role in the artisanal cheeses microenvironment. A phylogenetic analysis of rRNA 16S gene f...
Source: Food Research International - Category: Food Science Source Type: research
Authors: Okamura T, Tamura M, Suguro H, Ohtsu M, Omagari D, Yoshino A, Ogiso B, Asano M Abstract Sodium hypochlorite (NaOCl) is widely used as an antimicrobial irrigant; however, it has cytotoxic and neurotoxic effects. For these reasons, development of new, safe irrigants other than NaOCl is long overdue. In the present study, the antimicrobial and noxious effects of acid-electrolyzed functional water (FW) were evaluated and compared with those of NaOCl. Enterococcus faecalis, Streptococcus mutans, Porphyromonas gingivalis, or Candida albicans were mixed with each tested solution for 30 s. The mixtures were then p...
Source: Journal of oral science - Category: Dentistry Tags: J Oral Sci Source Type: research
Vancomycin-resistant enterococci are troublesome pathogens in clinical settings because of few treatment options. A VanA/VanM-type vancomycin-resistant Enterococcus faecium clinical isolate was identified in Japan. This strain, named AA708, harbored five plasmids, one of which migrated during agarose gel electrophoresis without S1 nuclease treatment, which is indicative of a linear topology. We named this plasmid pELF1. Whole genome sequencing (WGS) analysis of the AA708 strain revealed that the complete sequence of pELF1 was 143,316 bp long and harbored both the vanA and vanM gene clusters. Furthermore, mfold analysis and...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Nature, Published online: 13 November 2019; doi:10.1038/s41586-019-1742-xIn patients with alcoholic hepatitis, cytolysin-positive Enterococcus faecalis strains are correlated with liver disease severity and increased mortality, and in mouse models these strains can be specifically targeted by bacteriophages.
Source: Nature AOP - Category: Research Authors: Source Type: research
In this report, a 60 years old woman with a past medical history of significant chronic kidney disease undergoing regular hemodialysis, chronic constipation and hepatitis B was admitted to our neurology clinic because of acute intracerebral hemorrhage. She had confusion and right hemiplegia in her neurological examination and required urinary catheterization due to immobilization. Red coloration was observed in urine on the tenth hospital day. Although this coloration was thought to be hematuria, according to urine examination it was not hematuria. Then urine color turned into purple within two days. The next day, because ...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
This study explored the bacterial diversity of 64 spoiled custard cream desserts manufactured by 2 French companies. B. cereus group bacteria, coagulase negative Staphylococcus, Enterococcus and Leuconostoc spp. were isolated from spoiled products. Thirty-one bacterial isolates representative of the main spoilage species were tested for their spoilage abilities. Significant growth and pH decrease were observed regardless of species. While off-odours were detected with B. cereus group and staphylococci, yoghurt odours were detected with Enterococcus spp. and Leuconostoc spp. B. cereus group bacteria produced various esters ...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
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