Therapeutic effects of calcitonin gene-related peptide-modified bone marrow mesenchymal stem cells combined with autogenous bone grafting for treatment of osteonecrosis of the femoral head in rabbits

Publication date: Available online 14 October 2019Source: Biochemical Engineering JournalAuthor(s): Jinfeng Li, Guowei Shang, Yisheng Wang, Xiuli Wang, Yuanyuan Ji, Yuebai LiAbstractCalcitonin gene-related peptide (CGRP) promotes proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), which influence bone regeneration and reconstruction. We previously established CGRP-modified BMSCs that express the CGRP gene and demonstrated that this elevated CGRP expression promotes osteogenic differentiation of BMSCs. The current study investigated the therapeutic effects of CGRP-modified BMSCs combined with autogenous bone grafting for the treatment of osteonecrosis of the femoral head (ONFH) in rabbits. ONFH model rabbits were divided into three groups: CGRP, BMSCs/pLV5-CGRP combined with autogenous bone grafting; NC, BMSCs/pLV5-NC combined with autogenous bone grafting; and Blank, BMSCs combined with autogenous bone grafting. After autogenous bone grafting, mRNA and protein expression levels of osteogenesis-related genes (CGRP, OCN, Runx2, BMP-2, and OPG) were measured in the femoral head, as were ALP, laminin, and collagen type I contents. The fraction of trabecular bone area, percentage of empty osteocyte lacunae, maximum compressive strength, and mean elasticity modulus of the femoral head were also determined. Our results demonstrated that CGRP-modified BMSCs combined with autogenous bone grafting accelerated osteogenic differentiation of BMSCs, ...
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research