Therapeutic effects of calcitonin gene-related peptide-modified bone marrow mesenchymal stem cells combined with autogenous bone grafting for treatment of osteonecrosis of the femoral head in rabbits

Publication date: Available online 14 October 2019Source: Biochemical Engineering JournalAuthor(s): Jinfeng Li, Guowei Shang, Yisheng Wang, Xiuli Wang, Yuanyuan Ji, Yuebai LiAbstractCalcitonin gene-related peptide (CGRP) promotes proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), which influence bone regeneration and reconstruction. We previously established CGRP-modified BMSCs that express the CGRP gene and demonstrated that this elevated CGRP expression promotes osteogenic differentiation of BMSCs. The current study investigated the therapeutic effects of CGRP-modified BMSCs combined with autogenous bone grafting for the treatment of osteonecrosis of the femoral head (ONFH) in rabbits. ONFH model rabbits were divided into three groups: CGRP, BMSCs/pLV5-CGRP combined with autogenous bone grafting; NC, BMSCs/pLV5-NC combined with autogenous bone grafting; and Blank, BMSCs combined with autogenous bone grafting. After autogenous bone grafting, mRNA and protein expression levels of osteogenesis-related genes (CGRP, OCN, Runx2, BMP-2, and OPG) were measured in the femoral head, as were ALP, laminin, and collagen type I contents. The fraction of trabecular bone area, percentage of empty osteocyte lacunae, maximum compressive strength, and mean elasticity modulus of the femoral head were also determined. Our results demonstrated that CGRP-modified BMSCs combined with autogenous bone grafting accelerated osteogenic differentiation of BMSCs, ...
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research

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ConclusionThe finding suggested that LC had the ability to protect neurons by promoting the endogenous proliferation of neuroblast and production of neural differentiation factor in rats after ischemia injury. Meanwhile, LC can anti-neuroinflammation, which is important for the treatment of neuron injury. Accordingly, LC perhaps a promising medicine for neuron damage therapy after cerebral ischemia.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
We present a reproducible knock-in strategy combined with a highly specific homologous directed repair (HDR) screening approach using digital droplet PCR (ddPCR). The knock-in cell lines that we created show a functioning fluorescent reporter system for DA neurons that are identifiable by flow cytometry.
Source: Stem Cell Research - Category: Stem Cells Source Type: research
In conclusion, high-dose NR induces the onset of WAT dysfunction, which may in part explain the deterioration of metabolic health. Towards a Rigorous Definition of Cellular Senescence https://www.fightaging.org/archives/2019/11/towards-a-rigorous-definition-of-cellular-senescence/ The accumulation of lingering senescent cells is a significant cause of aging, disrupting tissue function and generating chronic inflammation throughout the body. Even while the first senolytic drugs capable of selectively destroying these cells already exist, and while a number of biotech companies are working on the productio...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Publication date: Available online 7 November 2019Source: CellAuthor(s): Yi Wang, I-Ling Chiang, Takahiro E. Ohara, Satoru Fujii, Jiye Cheng, Brian D. Muegge, Aaron Ver Heul, Nathan D. Han, Qiuhe Lu, Shanshan Xiong, Feidi Chen, Chin-Wen Lai, Hana Janova, Renee Wu, Charles E. Whitehurst, Kelli L. VanDussen, Ta-Chiang Liu, Jeffrey I. Gordon, L. David Sibley, Thaddeus S. StappenbeckSummaryThe colonic epithelium can undergo multiple rounds of damage and repair, often in response to excessive inflammation. The responsive stem cell that mediates this process is unclear, in part because of a lack of in vitro models that reca...
Source: Cell - Category: Cytology Source Type: research
CONCLUSIONS: PLGA and sIL-6R-treated MSCs help to repair articular cartilage with high efficacy. Thus, the application of this promising strategy to regenerative medicine for articular cartilage in patients with RA is anticipated. PMID: 31694744 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Rheumatology - Category: Rheumatology Tags: Clin Exp Rheumatol Source Type: research
Publication date: Available online 7 November 2019Source: Carbohydrate PolymersAuthor(s): Zahra Mahmoudi, Javad Mohammadnejad, Sajad Razavi Bazaz, Ali Abouei Mehrizi, Masoud Saidijam, Rassoul Dinarvand, Majid Ebrahimi Warkiani, Masoud SoleimaniAbstractThe field of cartilage tissue engineering has been evolved in the last decade and a myriad of scaffolding biomaterials and bioactive agents have been proposed. Controlled release of growth factors encapsulated in the polymeric nanomaterials has been of interest notably for the repair of damaged articular cartilage. Here, we proposed an on-chip hydrodynamic flow focusing micro...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Response of spinal cord ependymal stem cells to spinal cord injury. A, Glutamate stimulates neural stem/progenitor cell (NSPC) proliferation and enhances survival through AMPA receptor activation. B, M1 macrophages increase pro ‐inflammatory cytokine mRNA expression in NSPCs and enhance proliferation through MAPK‐Sox2 signaling. M2 macrophages increase anti‐inflammatory cytokine mRNA expression in NSPCs, reduce proliferation, and increase neuronal differentiation. C, Myelin‐derived inhibitory proteins limit NSPC pr oliferation and neuronal differentiation via NgR1 signaling. D, NSPCs maintain a high endogenous leve...
Source: Stem Cells - Category: Stem Cells Authors: Tags: TISSUE ‐SPECIFIC STEM CELLS Source Type: research
AbstractGenome editing of hematopoietic stem cells (HSCs) represents a therapeutic option for a number of hematological genetic diseases, as HSCs have the potential for self ‐renewal potential and differentiation into all blood cell lineages. This review presents advances of genome editing in HSCs utilizing adenovirus vectors as delivery vehicles. We focus on capsid‐modified, helper‐dependent adenovirus vectors that are devoid of all viral genes and therefore exhi bit an improved safety profile. We discuss HSC genome engineering for several inherited disorders and infectious diseases including hemoglobinopathies, Fan...
Source: FEBS Letters - Category: Biochemistry Authors: Tags: REVIEW Source Type: research
Response of spinal cord ependymal stem cells to spinal cord injury. A, Glutamate stimulates neural stem/progenitor cell (NSPC) proliferation and enhances survival through AMPA receptor activation. B, M1 macrophages increase pro ‐inflammatory cytokine mRNA expression in NSPCs and enhance proliferation through MAPK‐Sox2 signaling. M2 macrophages increase anti‐inflammatory cytokine mRNA expression in NSPCs, reduce proliferation, and increase neuronal differentiation. C, Myelin‐derived inhibitory proteins limit NSPC pr oliferation and neuronal differentiation via NgR1 signaling. D, NSPCs maintain a high endogenous leve...
Source: Stem Cells - Category: Stem Cells Authors: Tags: TISSUE ‐SPECIFIC STEM CELLS Source Type: research
CONCLUSIONS: The combined treatment induced strongest signals related to angiogenesis, bone formation, and tendon generation in-situ. We demonstrated that the combination of MSCs and PRP synergistically promotes tendon-bone healing and holds great promise for the treatment of rotator cuff injuries. PMID: 31696498 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
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