Biofabrication of multiscale bone extracellular matrix scaffolds for bone tissue engineering.

Biofabrication of multiscale bone extracellular matrix scaffolds for bone tissue engineering. Eur Cell Mater. 2019 Oct 11;38:168-187 Authors: Freeman FE, Browe DC, Nulty J, Von Euw S, Grayson WL, Kelly DJ Abstract Interconnected porosity is critical to the design of regenerative scaffolds, as it permits cell migration, vascularisation and diffusion of nutrients and regulatory molecules inside the scaffold. 3D printing is a promising strategy to achieve this as it allows the control over scaffold pore size, porosity and interconnectivity. Thus, the aim of the present study was to integrate distinct biofabrication strategies to develop a multiscale porous scaffold that was not only mechanically functional at the time of implantation, but also facilitated rapid vascularisation and provided stem cells with appropriate cues to enable their differentiation into osteoblasts. To achieve this, polycaprolactone (PCL) was functionalised with decellularised bone extracellular matrix (ECM), to produce osteoinductive filaments for 3D printing. The addition of bone ECM to the PCL not only increased the mechanical properties of the resulting scaffold, but also increased cellular attachment and enhanced osteogenesis of mesenchymal stem cells (MSCs). In vivo, scaffold pore size determined the level of vascularisation, with a larger filament spacing supporting faster vessel in-growth and more new bone formation. By freeze-drying solubilised bone ECM wi...
Source: European Cells and Materials - Category: Cytology Tags: Eur Cell Mater Source Type: research