GSE138801 Sequencing of the 3' end of RNA using BRB-seq in Drosophila heterozygous cross

Contributors : Michael V Frochaux ; Maroun B Sleiman ; Bart DeplanckeSeries Type : Expression profiling by high throughput sequencingOrganism : Drosophila melanogasterResistance to enteric pathogens is a complex trait at the crossroads of multiple biological processes. We have previously shown in the Drosophila Genetic Reference Panel (DGRP) that resistance to infection is highly heritable, but our understanding of how the effects of genetic variants are channeled through distinct molecular layers is still limited. To address this, we employed a systems genetics approach on the gut transcriptomes from 38 control and orally-infected DGRP lines, identifying a large number of condition-specific expression quantitative trait loci (cis-eQTLs). By assessing the allelic imbalance in the transcriptomes of 19 F1 hybrid lines from a large round-robin design, we could independently attribute a robust cis-regulatory effect to only 10% of the detected cis-eQTLs. These results therefore suggest that many context-dependent eQTLs that are assumed to act in cis, may act in trans instead. Further comparison of the transcriptomes of resistant and susceptible DGRP lines revealed Nutcracker (ntc) as the only differentially expressed gene between resistance classes. Interestingly, we found that ntc is linked to infection-specific eQTLs that not only correlate with its expression level, but also to enteric infection susceptibility. This is consistent with our findings that ntc expression is induced...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Drosophila melanogaster Source Type: research

Related Links:

There's new evidence that the diversity of bacteria, virus and other microbes in the gut affects a patient's odds of a successful stem cell transplant. It's the latest study in a growing body of research showing the microorganisms quietly thriving in our intestines affect our health in a variety of ways.Reuters Health Information
Source: Medscape Transplantation Headlines - Category: Transplant Surgery Tags: Transplantation News Source Type: news
Authors: Nadeem O, Anderson KC Abstract Introduction: Multiple myeloma therapy has evolved significantly over the past several decades due to discovery of numerous novel agents. These targeted agents are highly effective, but differ in their toxicity profiles. This literature review will highlight the safety of current and emerging myeloma therapy.Areas covered: Therapeutic agents that are reviewed for safety and efficacy data include alkylators, proteasome inhibitors, immunomodulatory agents, monoclonal antibodies, nuclear export inhibitor, and histone deacetylase inhibitor. Emerging studies of BCL2 inhibitors and...
Source: Expert Opinion on Drug Safety - Category: Drugs & Pharmacology Tags: Expert Opin Drug Saf Source Type: research
To evaluate the feasibility of seeding and growing rat mesenchymal stem cells on polypropylene mesh, to allow for future study of stem cell impact on tissue integration of implanted mesh.
Source: American Journal of Obstetrics and Gynecology - Category: OBGYN Authors: Tags: Oral Presentation Source Type: research
Authors: Nguyen MP, Jain V, Iansante V, Mitry RR, Filippi C, Dhawan A Abstract IntroductionHepatocyte transplantation (HT) is a promising alternative to liver transplantation for treatment of liver-based metabolic diseases and acute liver failure (ALF). However, shortage of good-quality liver tissues, early cell loss post-infusion, reduced cell engraftment and function restricts clinical application.Areas covered: A comprehensive literature search was performed to cover pre-clinical and clinical HT studies. The review discusses the latest developments to address HT limitations: cell sources from marginal/suboptimal...
Source: Expert Review of Gastroenterology and Hepatology - Category: Gastroenterology Tags: Expert Rev Gastroenterol Hepatol Source Type: research
Contributors : Jennifer Hammelman ; Richard SherwoodSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusHere we introduce the Multiplexed Integrated Accessibility Assay (MIAA), a multiplexed parallel reporter assay which measures changes to genome accessibility as a result of the integration of synthetic oligonucleotide phrase libraries into a controlled, natively inaccessible genomic context. We apply MIAA to measure the effects of sequence motifs on cell type-specific DNA accessibility between mouse embryonic stem cells and embryonic stem cell-derived definitive endoderm c...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research
Contributors : Lina Sui ; Yurong Xin ; Daniela Georgieva ; Giacomo Diedenhofen ; Leena Haataja ; Yong Wang ; Qi Su ; Jinrang Kim ; Jiayu Fu ; Danielle Baum ; Robin S Goland ; Peter Arvan ; Sandra Kleiner ; Dieter EgliSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCell cycle progression plays an important role in mediating the transition from a differentiated state to a pluripotent stem cell. Conversely, establishing limitations in proliferative potential may be important to achieve functional maturity as well as to prevent abnormal growths after transplantation of stem cell derived p...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Pierre Osteil ; Patrick P TamSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe modulation of TGFb activity is critical for the specification of mesendoderm tissue progenitors in the multipotent early epiblast and the repression of TGFb activity enhances the ectoderm lineage potency of the epiblast cells.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Contributor : Seba AlmedawarSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRPE was derived from hESC based on the protocol described in Zhu et al. PlosOne. 2013.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
This study is a multicenter, open-label trial to examine the effectiveness and safety of transplantation with reduced-intensity conditioning (RIC) for patients under 20 years old with relapsed or refractory ALCL. We defined RIC as the administration of fludarabine (30 mg/m2/day) for five days plus melphalan (70 mg/m2/day) for two days and total body irradiation at 4 Gy, followed by allogeneic hematopoietic stem cell transplantation. PMID: 32099255 [PubMed - in process]
Source: Acta Medica Okayama - Category: General Medicine Tags: Acta Med Okayama Source Type: research
Authors: Chen Y, Ohara T, Xing B, Qi J, Noma K, Matsukawa A Abstract Iron is a trace but vital element in the human body and is necessary for a multitude of crucial processes in life. However, iron overload is known to induce carcinogenesis via oxidative stress. Cancer cells require large amounts of iron for their rapid division and cell growth. Iron was recently found to play a role in cancer stem cells (CSCs); it maintains stemness during development. Iron also plays an important role in stemness by moderating reactive oxygen species. Thus, iron metabolism in CSCs is a promising therapeutic target. In this review...
Source: Acta Medica Okayama - Category: General Medicine Tags: Acta Med Okayama Source Type: research
More News: Genetics | Molecular Biology | Stem Cell Therapy | Stem Cells