Biotechnological potential of exopolysaccharide as a bioemulsifier produced by Rhizobium radiobacter CAS isolated from curd

Publication date: Available online 11 October 2019Source: Bioactive Carbohydrates and Dietary FibreAuthor(s): Digambar Kavitake, Farhat Zakirhusain Marchawala, Cedric Delattre, Prathapkumar Halady Shetty, Hilor Pathak, Prasad AndhareAbstractSuccinoglycan exopolysaccharide (EPS) from Rhizobium radiobacter CAS and its rheological behavior has been studied earlier. In present investigation the emulsifying potential of CAS EPS has been determined. CAS EPS concentration was optimized with hydrocarbons soybean oil and n-Hexane, whereas effect of pH (3.0–11.0), salinity, monovalent ions, divalent ion, (NaCl, KCl and MgCl2), freezing/thawing, temperature heating, emulsion stability and rheology on emulsifying activity (EA) was inspected using n-Hexane, paraffin oil, groundnut oil and soybean oil. CAS biopolymer represented superior emulsion stabilization ability (100%) under stress conditions and was highly tolerant to extreme pH, inorganic salts concentrations. Thermal stability of emulsions has found 100% at −20 °C–40 °C, whereas, at temperature 50 °C–120 °C high emulsion index (82.4–94.1% respectively) were recorded. Wetting filter paper test verified oil-in-water (o/w) type emulsion whereas rheological property showed pseudoplastic nature of emulsions. During stability of emulsions up to 30 days, some reduction of emulsification activity was observed for paraffin oil, however CAS hydrocolloid formed 100% emulsion index for n-Hexane, groundnut and soybean oil...
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research