Genes, Vol. 10, Pages 796: Characterization of GLOD4 in Leydig Cells of Tibetan Sheep During Different Stages of Maturity

Genes, Vol. 10, Pages 796: Characterization of GLOD4 in Leydig Cells of Tibetan Sheep During Different Stages of Maturity
Genes doi: 10.3390/genes10100796
Authors:
Wang
Li
Liu
Zhang
Zhao
Ma

We have previously reported that glyoxalase domain-containing protein 4 (GLOD4) is expressed in sheep testes by proteome analysis, but its roles during testicular development remain unclear. The aim of this study was to understand the expression characteristics and biological functions of the GLOD4 gene in developmental Tibetan sheep testes. The cDNA sequence of the Tibetan sheep GLOD4 gene was cloned by the RT-PCR method, and the structural characteristics of the GLOD4 protein were analyzed using relevant bioinformatics software, including ProtParam, TMHMM, Signal P 4.1, SOPMA, and phyre2. The expression patterns and immunolocalization of GLOD4 were examined in developmental testes derived from three-month-old (3M), one-year-old (1Y), and three-year-old (3Y) Tibetan sheep using quantitative real-time PCR (qRT-PCR), Western blot, immunohistochemistry, and immunofluorescence staining. The sequence analysis showed that the coding sequence (CDS) region of the GLOD4 gene was 729 bp in length and encoded 242 amino acids. Bioinformatics analysis found that the nucleotide and amino acid sequence of Tibetan sheep GLOD4 exhibited the highest sequence similarity with goat and chiru, and the least with zig-zag eel, of the species compared. GLOD4 expressions at both the mRNA an...

Source: Genes - October 11, 2019 Category: Genetics & Stem Cells Authors: Wang Li Liu Zhang Zhao Ma Tags: Article Source Type: research