The Significance of IgG to Aspergillus in Non-Cystic Fibrosis Bronchiectasis
The Significance of IgG to Aspergillus in Non-Cystic Fibrosis BronchiectasisConference abstracts
Conclusions. The applied diagnostic criteria had no significant effect on the reported rate of ABPA prevalence. PMID: 31702121 [PubMed - as supplied by publisher]
In this study we combine genetic approaches and GC-MS-mediated volatile analysis to show that A. fumigatus assimilates VSCs via cysteine (CysB)- or homocysteine (CysD)-synthase. This process is essential for utilization of VSCs as sulfur sources, since P. aeruginosa-derived VSCs trigger growth of A. fumigatus wild-type, but not of a ΔcysBΔcysD mutant, on sulfur-limiting media. P. aeruginosa produces VSCs when infecting Galleria mellonella and co-infection with A. fumigatus in this model results in a synergistic increase in mortality and of fungal and bacterial burdens. Interestingly, the increment in mortality ...
The clinical effects of Aspergillus fumigatus in the cystic fibrosis (CF) airway, with the exception of allergic bronchopulmonary aspergillosis, is unclear.
Publication date: Available online 3 August 2019Source: Journal of Microbiological MethodsAuthor(s): Emma Reece, Siobhán McClean, Peter Greally, Julie RenwickAbstractAspergillus fumigatus is the most common fungus infecting/colonising people with cystic fibrosis (CF) and can negatively impact clinical status. Diagnostic laboratories rely on culture to detect A. fumigatus which is known to be less sensitive than molecular approaches. Therefore, A. fumigatus colonisation in the CF population may be underestimated.Sputum (n = 60) from 25 children with CF were collected and A. fumigatus was detected using routine cu...
In conclusion, reliable identification of Aspergillus species is achieved by using molecular sequencing, especially for the emerging rare and cryptic species. They are mostly indistinguishable by conventional methods and might exhibit variable antifungal susceptibility profiles. Moreover, investigation of the antifungal susceptibility patterns is necessary for improved antifungal therapy against aspergillosis.
Nontuberculous mycobacteria (NTM) cause difficult-to-treat opportunistic infections, most frequently of the lungs. Patients with chronic obstructive pulmonary diseases, cystic fibrosis or bronchiectasis are prone to NTM pulmonary disease (PD) and other opportunistic infections, including by Aspergillus fumigatus. Co-infections are difficult to identify as diagnostic criteria for NTM-PD and chronic pulmonary aspergillosis (CPA) overlap [1, 2]. The literature suggests that NTM and Aspergillus co-infections are associated with higher mortality . Therefore, Aspergillus serology is part of NTM-PD diagnostic work up in our reference centre.