Imaging of bioluminescent Acinetobacter baumannii in a mouse pneumonia model
Publication date: December 2019Source: Microbial Pathogenesis, Volume 137Author(s): Seok Hyeon Na, Man Hwan Oh, Hyejin Jeon, Yun-Ki Lee, Byungheon Lee, Minsang Shin, Je Chul LeeAbstractBioluminescence imaging is a non-invasive tool for in vivo real-time monitoring of infectious disease progression in animal models. However, no bioluminescence imaging assay has been developed to monitor Acinetobacter baumannii infections. In the current study, bioluminescent strains of A. baumannii ATCC 17978 and its isogenic ΔompA mutant were constructed by integrating the promoter of the ompA gene and the luxCDABE luciferase gene into the bacterial chromosome. In an acute murine pneumonia model, bioluminescence of the two reporter strains was clearly visible in the lungs and the bioluminescent signal increased over time. Bioluminescence was correlated with bacterial burden and histopathology in reporter strain-infected mice, suggesting that bioluminescent bacteria are useful for monitoring A. baumannii infections in animal models.
ConclusionsThis study shows that the alveolar microbiota profile in BAL samples from TB and IP patients, representing infectious and non-infectious lung diseases, respectively, is characterized by decreased diversity.
Conclusion: The presence of XDR strains of A. baumannii is considerable significant problem in hospitals. Further, similar genetic clonal identified in them indicated the nosocomial infection origin. Hence, these results are very important for control of nosocomial infection committee in health-care systems. PMID: 31673531 [PubMed]
Conclusions: SOFA score at admission, re-intubation, and history of previous hospitalization were independent predictors of VAP. Antimicrobial therapy for VAP should cover MDR Gram-negative organisms.
ConclusionsIH colistin provided good outcomes with few side effects, and appropriate dosing of IV colistin was important to avoid excess mortality.
Authors: Panchal G, Pandit R, Trailokya A, Sharma A Abstract Antibiotic resistance is one of the biggest menace to global health. Deaths from Drug-resistant infections is set to escalate exponentially. Pipeline for new antibacterials is almost empty. The World Health Organization has reinforced its warning that to tackle growing threat of antimicrobial resistance, development of a new antibiotics is seriously lacking. Arbekacin is a novel aminoglycoside primarily used in the treatment of infections caused by resistant Staphylococcus Aureus i.e. Methicillin Resistant Staphylococcus Aureus (MRSA). Besides MRSA it als...
Hospital-acquired pneumonia (HAP) is a lung infection that develops after more than 48 hours of hospitalization, and, if associated with mechanical ventilation, it is termed ventilator-associated pneumonia (VAP).1 They are the second most common nosocomial infections and are important causes of morbidity and mortality around the world.2,3 These nosocomial pneumonias increase duration of hospitalization and healthcare costs. A matched case-control study demonstrated that having VAP prolonged duration of mechanical ventilation, intensive care unit (ICU) stay and hospitalization.
CONCLUSIONS: IH colistin provided good outcomes with few side effects, and appropriate dosing of IV colistin was important to avoid excess mortality. PMID: 31607573 [PubMed - as supplied by publisher]
This study was performed in 24 centers between January 06, 2014, and December 02, 2016. The patients were evaluated at time of pneumonia diagnosis, when culture results were available, and at 72 h, at the 7th day, and finally at the 28th day of follow-up. Patients with coexistent infections were excluded and only those with a first VAP episode were enrolled. Logistic regression analysis was performed. A total of 177 patients were included; empiric antimicrobial therapy was appropriate (wh en the patient received at least one antibiotic that the infecting strain was ultimately shown to be susceptible) in only 69 (39%)...
Abstract Candida auris is an emerging pathogenic yeast responsible for nosocomial infections with high mortality, on a global scale. A 65-year-old woman with hypovolemic shock and severe metabolic acidosis was intubated and admitted to the intensive care unit (ICU). Shortly after admission, she developed ventilator-associated pneumonia caused by multidrug-resistant Acinetobacter baumannii, which necessitated treatment with high-dose ampicillin-sulbactam. Two weeks later, a yeast was cultured from her blood. It formed pale pink colonies on CHROMagar Candida medium and produced predominantly oval budding yeast cells...