Improvement of Lactobacillus plantarum for the enhanced production of bacteriocin like inhibitory substance using combinatorial approach

Publication date: Available online 10 October 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Fariha Ibrahim, Syeda Bushra Zafar, Afsheen Aman, Shah Ali Ul Qader, Asma AnsariAbstractOver the last few decades, emerging cases of chemical additives and food borne pathogens have enforced the demand of natural antimicrobial compounds. Bacteriocins are one of the natural alternatives to the chemical food preservatives. The research on new and improved bacteriocins is in full swing due to some limitations of commercially available bacteriocins. The aim of the study is to improve the indigenously isolated strain for the enhanced production of bacteriocin like inhibitory substance (BLIS) using combinatorial approach. In the current study, the efficiency of BLIS produced by Lactobacillus plantarum KIBGE-IB45 was improved by applying a combinatorial strategies. Primarily, production of BLIS was optimized using one factor at a time approach. To further enhance the potential of L. plantarum, chemical mutagens were used followed by statistical optimization using multivariate approach. The results revealed that synergistic effect of chemical mutagen and a sequential statistical optimization have significantly enhanced the production of BLIS at 35 °C in MRS medium with an initial pH 9.0 after 16 h of incubation. Response surface methodology revealed 2.0 fold increased in the production of BLIS. The ability of BLIS to inhibit the Listeria monocytogenes ATCC 7644 has ...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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In this study, a collection of isolates from foods and food processing environments (FPEs) representing persistent, prevalent, and rarely detected genotypes was evaluated for biofilm forming capacities including adhesion and sessile biomass production under diverse environmental conditions. The quantity of sessile biomass varied according to growth conditions, lineage, serotype as well as genotype but association of clonal complex (CC) 26 genotype with biofilm production was evidenced under cold temperature. In general, relative biofilm productivity of each strain varied inconsistently across growth conditions. Under our e...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, CAP treatment was combined with a mild hydrogen peroxide (H2O2) treatment for disinfection of 1 and 7 day(s) old Listeria monocytogenes and Salmonella Typhimurium biofilms. Three different treatment sequences were investigated in order to determine the most effective treatment sequence, i.e., (i) first CAP, then H2O2, (ii) first H2O2, then CAP, and (iii) a simultaneous treatment of CAP and H2O2. Removal of the biofilm, induction of sub-lethal injury, and H2O2 breakdown due to the presence of catalase within the biofilms were investigated in order to comment on their possible contribution to the combined inac...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study demonstrates the capability of the filtration-based SERS platform for detecting Salmonella Enteritidis in various aqueous matrices such as distilled water and rinsing water from fresh produce with high selectivity and sensitivity. Graphical abstract. PMID: 31745614 [PubMed - as supplied by publisher]
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Tags: Anal Bioanal Chem Source Type: research
Conclusions: Infection of MDCK epithelial cells with Listeria monocytogenes significantly alters the transcriptome of the infected host cells when those are examined 24 h post infection. Both infected and uninfected cells in an infected well show significant transcriptomic changes compared to uninfected cells but also compared to each other. Most importnantly innate immunity related pathways are singificanlty upregulated for un infected cells in an infected well and to a lesser degree for infected cells as compared to cells not exposed to infection.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Canis lupus familiaris Source Type: research
The foodborne pathogen Listeria monocytogenes causes the potentially lethal disease listeriosis. Within food-associated environments, L. monocytogenes can persist for long periods and increase the risk of contami...
Source: BMC Microbiology - Category: Microbiology Authors: Tags: Research article Source Type: research
Foodborne Pathogens and Disease, Ahead of Print.
Source: Foodborne Pathogens and Disease - Category: Food Science Authors: Source Type: research
Toll-like receptors (TLR) trigger the immune system to mount a rapid innate response capable of protecting the host from a wide variety of bacterial and viral pathogens. There is interest in harnessing TLR agonists to reduce the susceptibility of at-risk populations to infection. However, the widespread prophylactic use of TLR agonists has been compromised by the need to administer them by parenteral injection. An exception is the TLR7/8 agonist R848, which can boost gastrointestinal and systemic immunity when administered orally. This work examines the effect of R848 on host susceptibility to Listeria monocytogenes in a m...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Microbial Immunity and Vaccines Source Type: research
Publication date: Available online 15 November 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Carolina Medina Amado, Carlos J. Minahk, Eduardo Cilli, Rafael G. Oliveira, Fernando G. DupuyAbstractThe mechanism of action of the anti-Listeria peptide enterocin CRL35 was studied with biophysical tools by using lipid mixtures that mimicked Gram-positive plasma membranes. Langmuir monolayers and infrared spectroscopy indicated that the peptide readily interacted with phospholipid assembled in monolayers and bilayers to produce a dual effect, depending on the acyl chains. Indeed, short chain mixtures wer...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research
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Source: Forbes.com Healthcare News - Category: Pharmaceuticals Authors: Source Type: news
Publication date: Available online 14 November 2019Source: Food ChemistryAuthor(s): Selina Brighina, Cristina Restuccia, Elena Arena, Rosa Palmeri, Biagio FallicoAbstractThe antibacterial activities of the dicarbonyl compounds glyoxal (GO), methylglyoxal (MGO), 3-deoxyglucosone (3-DG) were assessed against Gram-positive and Gram-negative pathogenic and food spoilage bacteria, both in agarised and liquid assay system. The kinetics of dicarbonyls’ degradation at different antimicrobial assay conditions were studied, to determine the possible interference of the nutrient medium.In agarised assay system, GO and MGO exhib...
Source: Food Chemistry - Category: Food Science Source Type: research
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