Analysis of Bio Active Composites and Antiviral Activity of Iresine herbstii Extracts against Newcastle Disease Virus In ovo

Publication date: Available online 11 October 2019Source: Saudi Journal of Biological SciencesAuthor(s): Rahat Andleeb, Asma Ashraf, Saima Muzammil, Shabana Naz, Farkhanda Asad, Tayyaba Ali, Rida Rafi, K.A. Al-Ghanim, F. Al-Misned, Z. Ahmed, Shahid MahboobAbstractThe study was implemented to actuate the qualitative and quantitative phyto constituents of Iresine herbstii extracts and its antiviral efficacy against avian ND virus. Among four tested solvents, the ethanolic extract of Iresine herbstii revealed the presence of highest quantity of all tested phytochemicals while petroleum ether extract showed the least. Folin-Ciocalteu method assessed the range of TPC extended from 81.01 ± 0.67 to 126.35 ± 0.45 µg GAE/mg. Acetonic extract showed the highest amount among all extracts and petroleum ether possessed the lower quantity. TFC ranged from 54.37 ± 0.45 to 88.12 ± 0.26 µg QE/mg followed by colorimetric method. From all extract ethanolic extract showed highest quantity and petroleum ether revealed the lower. HPLC analysis of ethanolic extract of I. herbstii confesses the presence six bioactive components by using the HP5-MS column. To check the antiviral potential of plants, different prepared treatments of plant extract and live virus were inoculated at 9 days old SPF embryonated chicken eggs. Results exposed that all plant extracts produce antiviral activity against NDV in ovo according to their potential and phytochemical profile. The highest survival rate was observ...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research